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Anti-PTEN PHOSPHO Antibody EP229
Also for PTEN (NM_000314)
|A phospho-specific peptide corresponding to residues surrounding Threonine 366 and Serine 370 of human PTEN was used as an immunogen. The antibody only detects PTEN phosphorylated on T366 or S370.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:50; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens phosphatase and tensin homolog (PTEN)|
|10q23del; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN1; TEP1|
|PTEN is a protein tyrosine phosphatase that acts as a tumor suppressor, as a lipid phosphatase that dephosphorylates the D3 position of phosphatidylinositol 3,4,5-trisphosphate and as an antagonist of the PI3k/AKT signaling pathway (1-3). PTEN structural domains includes an N-terminal phosphatase domain, a lipid binding C2 domain and a 50-amino acid C-terminal tail that contains a PDZ biding sequence. Phosphorylation of the tail suppresses the activity of PTEN by controlling its recruitment into the PTEN-associated complex (4). PTEN is phosphorylated in vitro on Threonine 366 and Serine 370 by glycogen synthase kindase 3 (GSK3) and casein kinase 2 (CK2) respectively. Prior phosphorylation of PTEN at Serine 370 by CK2 strongly increased its phosphorylation at Threonine 366 by GSK3, suggesting that the two may synergize. Generally, phosphorylation in the C-terminal tail of PTEN is thought to enhance stability and to decrease membrane localization and activity. However, phosphorylation at Threonine 366 is linked to destabilization of PTEN (5). |
Senescence and Autophagy
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Western blot - PTEN (phospho T366 + S370) antibody [EP229]; All lanes : Anti-PTEN (phospho T366 + S370) [EP229] antibody at 1/1000 dilution.Lane 1 : HeLa cell lysates, untreated.Lane 2 : HeLa cell lysates, treated with Calyculin A.Lysates/proteins at 10 µg per lane.Predicted band size : 47 kDa.Observed band size : 54 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PTEN (phospho T366 + S370) antibody [EP229]; ab109454 at 1/100 dilution staining PTEN in Human breast ductal carcinoma by Immunohistochemistry, Paraffin-embedded tissue.