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Anti-PTEN PHOSPHO Antibody EP229


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against PTEN Phospho (pT366/pS370) (clone EP229 ) (Phospho-Specific)
100ul $325 3 Weeks
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OriGene Data

ImmunogenA phospho-specific peptide corresponding to residues surrounding Threonine 366 and Serine 370 of human PTEN was used as an immunogen. The antibody only detects PTEN phosphorylated on T366 or S370.
Clone NameEP229 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:50; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
Predicted MW Explanation 47 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens phosphatase and tensin homolog (PTEN), transcript variant 1
Alternative Name10q23del; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN1; TEP1
Database LinkNP_000305
Entrez Gene 5728 Human
Entrez Gene 19211 Mouse
Entrez Gene 50557 Rat
FunctionPTEN is a protein tyrosine phosphatase that acts as a tumor suppressor, as a lipid phosphatase that dephosphorylates the D3 position of phosphatidylinositol 3,4,5-trisphosphate and as an antagonist of the PI3k/AKT signaling pathway (1-3). PTEN structural domains includes an N-terminal phosphatase domain, a lipid binding C2 domain and a 50-amino acid C-terminal tail that contains a PDZ biding sequence. Phosphorylation of the tail suppresses the activity of PTEN by controlling its recruitment into the PTEN-associated complex (4). PTEN is phosphorylated in vitro on Threonine 366 and Serine 370 by glycogen synthase kindase 3 (GSK3) and casein kinase 2 (CK2) respectively. Prior phosphorylation of PTEN at Serine 370 by CK2 strongly increased its phosphorylation at Threonine 366 by GSK3, suggesting that the two may synergize. Generally, phosphorylation in the C-terminal tail of PTEN is thought to enhance stability and to decrease membrane localization and activity. However, phosphorylation at Threonine 366 is linked to destabilization of PTEN (5).
Related PathwayPhosphataseDruggable Genome Inositol phosphate metabolismPhosphatidylinositol signaling systemp53 signaling pathwayFocal adhesionTight junctionPathways in cancerMore Pathways >>

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Western blot - PTEN (phospho T366 + S370) antibody [EP229]; All lanes : Anti-PTEN (phospho T366 + S370) [EP229] antibody at 1/1000 dilution.Lane 1 : HeLa cell lysates, untreated.Lane 2 : HeLa cell lysates, treated with Calyculin A.Lysates/proteins at 10 ug per lane.Predicted band size : 47 kDa.Observed band size : 54 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PTEN (phospho T366 + S370) antibody [EP229]; TA307725 at 1/100 dilution staining PTEN in Human breast ductal carcinoma by Immunohistochemistry, Paraffin-embedded tissue.


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