 |
|
|
|
Home All anti-PTEN antibodies
Anti-PTEN Phospho Antibody EP229
TrueMAB™ Antibodies - Made against Authentic Protein Antigens
* Buy one primary antibody, get 50% off a GAPDH loading control antibody.
Also for PTEN (NM_000314)
OriGene Data
| A phospho-specific peptide corresponding to residues surrounding Threonine 366 and Serine 370 of human PTEN was used as an immunogen. The antibody only detects PTEN phosphorylated on T366 or S370. |
| EP229 |
Rabbit IgG |
| Human |
|
| WB, IHC |
ICC: 1:100 - 250, IHC: 1:100 - 250, IP: 1:50, WB: 1:1,000 - 10,000, |
| Store at -20 C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt. |
| (Protein A or G Sepharose) |
Reference Data
| Homo sapiens phosphatase and tensin homolog (PTEN) |
| 10q23del; BZS; DEC; GLM2; MHAM; MMAC1; PTEN1; TEP1 |
| NP_000305 |
| PTEN is a protein tyrosine phosphatase that acts as a tumor suppressor, as a lipid phosphatase that dephosphorylates the D3 position of phosphatidylinositol 3,4,5-trisphosphate and as an antagonist of the PI3k/AKT signaling pathway (1-3). PTEN structural domains includes an N-terminal phosphatase domain, a lipid binding C2 domain and a 50-amino acid C-terminal tail that contains a PDZ biding sequence. Phosphorylation of the tail suppresses the activity of PTEN by controlling its recruitment into the PTEN-associated complex (4). PTEN is phosphorylated in vitro on Threonine 366 and Serine 370 by glycogen synthase kindase 3 (GSK3) and casein kinase 2 (CK2) respectively. Prior phosphorylation of PTEN at Serine 370 by CK2 strongly increased its phosphorylation at Threonine 366 by GSK3, suggesting that the two may synergize. Generally, phosphorylation in the C-terminal tail of PTEN is thought to enhance stability and to decrease membrane localization and activity. However, phosphorylation at Threonine 366 is linked to destabilization of PTEN (5). |
Focal Adhesion Senescence and Autophagy |
* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
WB Image
A. Western blot analysis on HeLa cell lysates using anti-Phospho-PTEN (pT366/S370) RabMAb .
IHC Image
B. Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma using anti-Phospho-PTEN RabMAb .
Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:
- HRP
- Biotin
- PE
- FITC
- DyLight® 488
- DyLight® 550
- DyLight® 594
- DyLight® 633
* DyLight is a trademark of Thermo-Fisher Scientific
Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.
Contact us with your conjugation/bulk request: antibody@origene.com or 888-267-4436 (Tech Support)
|
 |
