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Anti-CD14 Antibody EPR3653
Also for CD14 (NM_000591)
|A synthetic peptide corresponding to residues in human CD14 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IHC
||WB: 1:1000 - 1:5000; IHC-P: 1:500 - 1:1000; ICC: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens CD14 molecule (CD14), transcript variant 1|
|CD14 antigen; monocyte differentiation antigen CD14; CD14 molecule|
|CD14 is a glycophosphatidylinositol-anchored protein expressed on the surfaces of monocytes and plymorphonuclear leukocytes. It acts as a co-receptor for the detection of bacterial lipopolysaccharide (LPS) (1). CD14 cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial LPS. It acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (2). CD14 is expressed strongly on peripheral blood monocytes and macrophages, and weakly on the surface of granulocytes (1). |
Hematopoietic cell lineage
MAPK signaling pathway
Toll-like receptor signaling pathway
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Western blot - Anti-CD14 antibody [EPR3653]; All lanes : Anti-CD14 antibody [EPR3653] at 1/1000 dilution.Lane 1 : PBMC cell lysate.Lane 2 : Human tonsil cell lysate.Lane 3 : SW480 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat Anti-rabbit HRP at 1/2000 dilution.Predicted band size : 40 kDa.Observed band size : 53 kDa .
Other-Anti-CD14 antibody [EPR3653](ab133335); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653]; Immunohistochemical analysis of Formalin-fixed, paraffin- embedded Human placenta tissue labelling CD14 with ab133335 at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653]; Immunohistochemical analysis of Formalin-fixed, paraffin- embedded Human tonsil tissue labelling CD14 with ab133335 at 1/500 dilution.