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Home Antibody All anti-NQO1 antibodies

Anti-NQO1 Antibody EPR3309

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307661
  • Rabbit monoclonal antibody against NQO1 (clone EPR3309 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400327) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
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Also for NQO1 (NM_000903)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the N-terminus of human NQO1 was used as an immunogen.
Clone NameEPR3309 IsotypeTissue culture supernatant
Species ReactivityHuman, Mouse ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:50; ICC: 1:100 - 1:250; IHC-P: Use at an assay dependent dilution
BufferDoes not react with Rat. Is unsuitable for Flow Cyt.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens NAD(P)H dehydrogenase, quinone 1 (NQO1), transcript variant 1
Alternative NameDHQU; DIA4; DTD; NMOR1; NMORI; QR1
Database LinkNP_000894
FunctionNQO1 (Quinone 1) is a flavoenzyme that catalyzes two-electron reductive metabolism and detoxification of quinones and their derivatives leading to protection of cells against redox cycling and oxidative stress (1). NQO1 is mainly cytosolic, but its nuclear presence suggests its involvement in both chemoprevention and bioactivation of DNA-damaging antitumor agents (2). NQ01 may also play a role in the bioactivation of antitumor quinones such as mitomycin C (MMC), and has been proposed to have a protective effect against cancer caused by quinones and their metabolic precursors (3-4).
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - NQO1 antibody [EPR3309]; All lanes : Anti-NQO1 antibody [EPR3309] at 1/50000 dilution.Lane 1 : MCF7 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : A549 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 31 kDa.Observed band size : 31 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody [EPR3309]; IHC image of TA307661 staining in human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with TA307661, undiluted, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

 

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