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Anti-NQO1 Antibody EPR3309
Also for NQO1 (NM_000903)
|A synthetic peptide corresponding to residues on the N-terminus of human NQO1 was used as an immunogen.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IP: 1:50; ICC: 1:100 - 1:250; IHC-P: Use at an assay dependent dilution
|Does not react with Rat. Is unsuitable for Flow Cyt.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt.
|Homo sapiens NAD(P)H dehydrogenase, quinone 1 (NQO1), transcript variant 1|
|DHQU; DIA4; DTD; NMOR1; NMORI; QR1|
|NQO1 (Quinone 1) is a flavoenzyme that catalyzes two-electron reductive metabolism and detoxification of quinones and their derivatives leading to protection of cells against redox cycling and oxidative stress (1). NQO1 is mainly cytosolic, but its nuclear presence suggests its involvement in both chemoprevention and bioactivation of DNA-damaging antitumor agents (2). NQ01 may also play a role in the bioactivation of antitumor quinones such as mitomycin C (MMC), and has been proposed to have a protective effect against cancer caused by quinones and their metabolic precursors (3-4). |
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Western blot - NQO1 antibody [EPR3309]; All lanes : Anti-NQO1 antibody [EPR3309] at 1/50000 dilution.Lane 1 : MCF7 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : A549 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 31 kDa.Observed band size : 31 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody [EPR3309]; IHC image of TA307661 staining in human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with TA307661, undiluted, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.