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Anti-CAPNS1 Antibody EPR3324
Also for CAPNS1 (NM_001003962)
|A synthetic peptide corresponding to residues in human Calpain 1 (small subunit) was used as an immunogen.|
|Mouse, Rat, Human, Chinese Hamster
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||FC: 1:50, ICC: 1:100 - 250, IHC: 1:100 - 250, WB: 1:1000 - 5000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens calpain, small subunit 1 (CAPNS1), transcript variant 2|
|30K; CALPAIN4; CANP; CANPS; CAPN4; CDPS; CSS1|
|Calcium-dependent cysteine proteinases, or calpains, are widely distributed in mammalian cells. There are 2 distinct molecular forms, calpains 1 and 2, which differ in the quantity of calcium required. Both calpains 1 and 2 are heterodimeric; each is composed of 1 heavy (about 80 kD), CAPN1 and CAPN2, respectively, and 1 shared light (about 30 kD) subunit, CAPNS1. The heavy subunit has a catalytic function, and the light subunit is regulatory (1). |
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Western blot - Calpain S1 antibody [EPR3324]; All lanes : Anti-Calpain S1 antibody [EPR3324] at 1/2000 dilution.Lane 1 : T47D cell lysate.Lane 2 : fetal brain lysate.Lane 3 : fetal spleen lysate.Lane 4 : 293T cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 28 kDa.Observed band size : 28 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calpain S1 antibody [EPR3324]; Immunohistochemistry staining of Calpain S1 in formalin-fixed, paraffin-embedded Human kidney tissue using 1/100 TA307652.
Flow Cytometry-Anti-Calpain S1 antibody [EPR3324](TA307652); Overlay histogram showing HeLa cells stained with TA307652 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.