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Anti-AKT1 PHOSPHO Antibody EPR4157
Also for AKT1 (NM_005163)
|A phospho specific peptide corresponding to residues surrounding threonine 450 of human Akt1 was used as an immunogen. This antibody only detects Akt1 phosphorylated at threonine 450.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or ICC.
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Western blot - AKT1 (phospho T450) antibody [EPR4157]; All lanes : Anti-AKT1 (phospho T450) antibody [EPR4157] at 1/1000 dilution.Lane 1 : NIH3T3 cell lysate, untreated .Lane 2 : NIH3T3 cell lysate, treated with Lambda Phosphatase.Lysates/proteins at 10 ug per lane.Predicted band size : 56 kDa.Observed band size : 60 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AKT1 (phospho T450) antibody [EPR4157]; TA307636 at 1/50 dilution staining AKT1 in Human breast carcinom by Immunohistochemistry, Paraffin-embedded tissue.