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Anti-AKT1 PHOSPHO Antibody EPR4157


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against Akt1 Phospho (pT450)(clone EPR4157) (Phospho-Specific)
100ul $325 3-7 Days
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OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding threonine 450 of human Akt1 was used as an immunogen. This antibody only detects Akt1 phosphorylated at threonine 450.
Clone NameEPR4157 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:50 - 1:100
Predicted MW Explanation 56 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or ICC.

Reference Data

Target NameHomo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 1
Database LinkNP_005154
Entrez Gene 207 Human
Entrez Gene 11651 Mouse
Entrez Gene 24185 Rat
FunctionAkt1/PKBa is a Serine/Threonine protein kinase whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth. Akt1/PKBa is activated by various growth and survival factors (1). Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3,4).
Related PathwayProtein KinaseES Cell Differentiation/IPSDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayApoptosisVEGF signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - AKT1 (phospho T450) antibody [EPR4157]; All lanes : Anti-AKT1 (phospho T450) antibody [EPR4157] at 1/1000 dilution.Lane 1 : NIH3T3 cell lysate, untreated .Lane 2 : NIH3T3 cell lysate, treated with Lambda Phosphatase.Lysates/proteins at 10 ug per lane.Predicted band size : 56 kDa.Observed band size : 60 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AKT1 (phospho T450) antibody [EPR4157]; TA307636 at 1/50 dilution staining AKT1 in Human breast carcinom by Immunohistochemistry, Paraffin-embedded tissue.


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