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Home Antibody All anti-HIST2H2BF antibodies

Anti-HIST2H2BF Antibody EP955Y

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SKU Description Amount Price Availability*  
TA307626
  • Rabbit monoclonal antibody against Histone H2B Acetyl (K15) (clone EP955Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC422516) , 20ug Explanation
100ul $325 3-7 Days
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WB(1)
IHC(1)
IF(1)
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Also for HIST2H2BF (NM_001024599)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic acetylated peptide corresponding to residues surrounding Lysine 15 of Histone H2B was used as an immunogen. The antibody only detects Histone H2B acetylated on Lysine 15.
Clone NameEP955Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF: 1:500; WB: 1:2000 - 1:5000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
Predicted MW Explanation 14 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens histone cluster 2, H2bf (HIST2H2BF), transcript variant 1
Alternative NameFLJ35099; FLJ56780; FLJ56787; MGC131639
Database LinkNP_001019770
Entrez Gene 440689 Human
FunctionChanges in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is primarily acetylated at lysines 5, 12, 15, and 20 (3). Ubiquitylation of Histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of apoptosis and meiosis (5).
Related Pathway Systemic lupus erythematosus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Histone H2B (acetyl K15) antibody [EP955Y]; All lanes : Anti-Histone H2B (acetyl K15) antibody [EP955Y] at 1/2000 dilution.Lane 1 : HeLa cell lysate (untreated).Lane 2 : HeLa cell lysate (treated with TSA).Lysates/proteins at 10 ug per lane.Secondary.Goat anti-Rabbit HRP conjugate at 1/2000 dilution.Predicted band size : 14 kDa.Observed band size : 17 kDa .
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H2B (acetyl K15) antibody [EP955Y]; Ab62335 (1/100-1/250) staining human Histone H2B in human breast carcinoma tissue by immunohistochemistry using paraffin embededd tissue.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K15) antibody [EP955Y]; TA307626 (1/500) staining Histone H2B (acetyl K15) in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red).
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