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Home Antibody All anti-ANXA5 antibodies

Anti-ANXA5 Antibody EPR3980

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307564
  • Rabbit monoclonal antibody against Annexin V(clone EPR3980)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400462) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IF(2)
FC(1)
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Also for ANXA5 (NM_001154)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Annexin V was used as an immunogen.
Clone NameEPR3980 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF, FC Suggested DilutionsWB: 1:10000 - 1:50000; FC: 1:100 - 1:1000; ICC/IF: 1:500 - 1:1000
Predicted MW Explanation 36 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IHC-P or IP.

Reference Data

Target NameHomo sapiens annexin A5 (ANXA5)
Alternative NameANX5; ENX2; HEL-S-7; PP4; RPRGL3
Database LinkNP_001145
Entrez Gene 308 Human
Entrez Gene 11747 Mouse
Entrez Gene 25673 Rat
FunctionAnnexin V (ANV) is a calcium-dependent glycoprotein with a potent anticoagulant capacity in vitro, mainly as a result of its negatively charged membrane phospholipids, and capable of inhibiting the prothrombinase and Tensa complexes to reduce plaque adhesion and aggregation. Circulating ANV can be released from the cells of the vascular wall (endothelial cells, smooth muscles cells) or from secretor cells of the spleen and liver. Once it is in the plasma, it binds to blood cells (platelets and erythrocytes) or to endothelial cells (1). Annexin V is also a Ca(2+) dependent membrane-binding protein protein that forms voltage-dependent Ca2+ channels in phospholipid bilayers (2). Structural features suggest that Annexin V attaches with its convex face to membrane by specific calcium mediated interactions with at least three phospholipids. The adjacent membrane bilayer may thus become locally disordered and permeable to allow calcium inflow through the central polar channel of the molecule.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Annexin V antibody [EPR3980]; All lanes : Anti-Annexin V antibody [EPR3980].Lane 1 : HeLa cell lysate.Lane 2 : Fetal kidney lysate.Lane 3 : Fetal brain lysate.Lane 4 : Jurkat cell lysate.Lane 5 : JAR cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 36 kDa.
IF Image
Immunocytochemistry/ Immunofluorescence - Annexin V antibody [EPR3980]; Immunofluorescent staining of HeLa cells using 1/500 TA307564.
IF Image
Immunocytochemistry/ Immunofluorescence - Annexin V antibody [EPR3980]; Immunofluorescent staining of staurosporin-treated apoptotic HeLa cells using 1/250 TA307564.
FC Image
Flow Cytometry - Anti-Annexin V antibody [EPR3980]; Overlay histogram showing HeLa cells stained with TA307564 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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