OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-XRCC6 antibodies

Anti-XRCC6 Antibody EPR4027

div

Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA307538
  • Rabbit monoclonal antibody against Ku70(clone EPR4027)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400567) , 20ug Explanation
100ul $325 3-7 Days
Add to Shopping Cart
spacer
WB(1)
IHC(1)
IF(1)
FC(1)
spacer
Also for XRCC6 (NM_001469)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Ku70 was used as an immunogen.
Clone NameEPR4027 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsFC: 1:10 - 100, ICC: 1:100 - 250, IHC: 1:100 - 250, IP: 1:10 - 100, WB: 1:1,000-10,000,
Predicted MW Explanation 70 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens X-ray repair complementing defective repair in Chinese hamster cells 6 (XRCC6), transcript variant 1
Alternative NameCTC75; CTCBF; G22P1; KU70; ML8; TLAA
Database LinkNP_001460
Entrez Gene 2547 Human
FunctionATP-dependent DNA helicase 2 subunit 1 (Ku70) is a single stranded DNA-dependent helicase originally recognized by the sera of patients with autoimmune diseases (1, 2). It plays a key role in multiple nuclear processes such as DNA repair, chromosome maintenance, transcription regulation, and V(D)J recombination (3). Ku70 forms a heterodimer with Ku80 and contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining (NHEJ) pathway (4). The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex, DNA-PK (1). Together, the Ku70/Ku80 heterodimer and DNA-PKcs are required for V(D)J recombination and DNA double-strand break repair and may also play a role in transcription regulation (5). In addition, the dimer associates with NARG1 to bind to the osteocalcin promoter and activate osteocalcin expression (1).
Related PathwayTranscription FactorsDruggable Genome Non-homologous end-joining

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Ku70 antibody [EPR4027]; All lanes : Anti-Ku70 antibody [EPR4027] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293T cell lysate.Lane 3 : A549 cell lysate.Lane 4 : A431 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 70 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ku70 antibody [EPR4027]; Paraffin embedded Human testis tissue (A) or Human tonsil tissue (B) were labelled with TA307538 at 1/100 dilution.
IF Image
Immunocytochemistry/ Immunofluorescence - Ku70 antibody [EPR4027]; Immunofluorescence staining of HeLa cells using TA307538 at 1/100 dilution.
FC Image
Flow Cytometry-Anti-Ku70 antibody [EPR4027](TA307538); Overlay histogram showing HeLa cells stained with TA307538 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

spacer
Inc 5000 Healthcare Company Copyright © 2015 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title