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Home Antibody All anti-ARF1 antibodies

Anti-ARF1 Antibody EPR443

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA307524
  • Rabbit monoclonal antibody against ARF1(clone EPR443)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419819) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(2)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human ARF1 was used as an immunogen.
Clone NameEPR443 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100
Predicted MW Explanation 21 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens ADP-ribosylation factor 1 (ARF1), transcript variant 4
Alternative NameOTTHUMP00000035715; ADP-ribosylation factor 1
Database LinkNP_001649
Entrez Gene 375 Human
Entrez Gene 11840 Mouse
Entrez Gene 64310 Rat
FunctionARF1 (small GTP-binding ADP-ribosylation factor 1) is a member of the ARF family, which contains five other ARF proteins (1). ARF1 functions in various membrane trafficking events in the ER-Golgi system and in the maintenance of organelle structure. Inactive ARF1 (ARF1-GDP) localizes in the cytoplasm, while the active form (ARF1-GTP) localizes in the membrane (2-3). Active ARF1 is also localized to the Golgi complex by regulating the assembly of coat proteins onto membranes and mediating traffic in the ER-Golgi. ARF1 activates phospholipase D and phosphatidylinositol 4-phosphate 5-kinase and recruits COPI to membranes (4-5).
Related Pathway Vibrio cholerae infection

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - ARF1 antibody [EPR443]; All lanes : Anti-ARF1 antibody [EPR443] at 1/1000 dilution.Lane 1 : Human fetal kidney lysate.Lane 2 : Human fetal brain lysate.Lane 3 : MCF7 lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 21 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ARF1 antibody [EPR443]; Immunohistochemical analysis of ARF1 in paraffin embedded Human breast ductal carcinoma tissue, using TA307524 at a dilution of 1/100.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ARF1 antibody [EPR443]; Immunohistochemical analysis of ARF1 in paraffin embedded Human colonic adenocarcinoma tissue, using TA307524 at a dilution of 1/100.

 

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