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Anti-GATA1 Antibody EP2819Y
Also for GATA1 (NM_002049)
|A synthetic peptide corresponding to N-terminus of human Gata-1 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:2000; FC: 1:50
|Does not react with Mouse, Rat. Is unsuitable for ICC/IF,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for ICC/IF,IHC-P or IP.
|Homo sapiens GATA binding protein 1 (globin transcription factor 1) (GATA1)|
|ERYF1; GATA-1; GF-1; GF1; NF-E1; NFE1; XLANP; XLTDA; XLTT|
|Globin transcription factor 1 (GATA-1) is a transcription activator belonging to the GATA family of transcription factors with importance in cell growth. GATA-1 is a Cys2-Cys2 zinc finger DNA binding protein commonly found in erthroid (red blood cell) and emagkaryotic (platelet producing cell) and it is critical in the development process of both (1). It binds to the consensus sequence of [AT]GATA[AG] within regulatory regions of globin genes and genes in erythroid cells, leading to transcription of alpha spectrin structural protein necessary for red blood cell’s shape. In human, GATA-1 can enhance transcription rate up to 100 times (2). Mutation to GATA-1 encoding gene has been implicated to X-linked dyserythropoietic anemia, thrombocytopenia, Down’s syndrome in children, congenital transient myeloproliferative syndrome, and acute megakaryocytic leukemia (2, 3). |
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Western blot - GATA1 antibody [EP2819Y]; Anti-GATA1 antibody [EP2819Y] at 1/2000 dilution + K562 cell lysate at 10 µg.Secondary.HRP labelled goat anti-rabbit at 1/1000 dilution.Predicted band size : 43 kDa.Observed band size : 43 kDa.
Flow Cytometry - GATA1 antibody [EP2819Y]; Flow cytometric analysis of permeabilized K562 cells using ab76121 at a 1/50 dilution (red) or a rabbit IgG (negative) (green).