A phospho specific peptide corresponding to residues surrounding tyrosine 1009 of human PDGF receptor beta was used as an immunogen. This antibody detects PDGFR-beta phosphorylated on Tyrosine 1009. This antibody is predicted to work in human samples, based on sequence analysis only.
Store at -20C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Human platelet-derived growth factor (PDGF) receptor is a receptor tyrosine kinase whose activity induces various signalling cascades leading to cell growth and proliferation. Two major isoforms exist, PDGF receptor-a and PDGF receptor-beta. PDGF receptor-beta can only bind two specific PDGF isoforms, PDGF-beta and PDGF-delta (1,2). Upon ligand binding, the receptor undergoes autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as PI kinase, Grb2, and Src, among others (3). It has been shown that SHP-2 and PLC-gamma bind to PDGF receptor-beta that is phosphorylated at Tyrosine 1009 (4). Both Tyrosine 751 and 740 is required for PDGFR-beta mediated PI3 kinase activation. In particular, Tyrosine 751 is the docking site for P13 kinase (5).
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