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Anti-PLCG1 Phospho Antibody EP1502Y
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Also for PLCG1 (NM_002660)
|A synthetic peptide corresponding to phospholyated residue surrounding tyrosine 1245 of human PLC?I was used.|
||ICC: 1: 100 - 250, WB: 1:2,000 - 5,000,
|Store at -20C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.|
|Homo sapiens phospholipase C, gamma 1 (PLCG1), transcript variant 1|
|NCKAP3; PLC-II; PLC1; PLC148; PLCgamma1|
|The phospholipase C gamma-1 (PLC?I) is a member of phospholipase C family which catalyzes the hydrolysis of phospholipids to yield second messengers such as diacylglycerols (DAG) and water-soluble phosphorylated derivatives of the lipid head groups. These second messengers play a crucial role in transmembrane signaling and are involved in the regulation of several cellular functions, such as cell differentiation and proliferation (1). PLC?I interacts with variety of signaling molecules via its two SH2 domains and a single SH3 domain and it is activated by phosphorylation on Tyr783 through interaction with non-receptor and receptor tyrosine kinases. Interaction with activated receptors (e.g. PDGFR and EGFR) leads to phosphorylation on Tyr771, Tyr783, and Tyr1245 (2). Phosphorylations at Tyr783 and 1245 might be significant for adhesion PLC?I to fibronectin during integrin initiated fibronectin adhesion (3) |
EGFR1 Signaling Pathway
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A. Western blot analysis on (1) Jurkat and (2) Jurkat + Pervanadate cell lysates using anti- PLC? I (pY1245) RabMAb (catalog #2125-1), 1:2,000 dilution.