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Anti-POU2F2 Antibody EPR542
Also for POU2F2 (NM_002698)
|A synthetic peptide corresponding to residues in human Oct.-2 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||FC: 1:50, ICC: 1:250 - 500, IHC: 1:250 - 500, WB: 1:1,000 - 5,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens POU class 2 homeobox 2 (POU2F2), transcript variant 2|
|Oct-2; OCT2; OTF2|
|Octamer transcription factor-2 (Oct-2) is a transcription factor that specifically binds to the octamer motif (5- ATTTCAT-3). Mostly expressed in B cells, Oct-2 posses a leucine zipper domain and belongs to the POU family of transcription factors (1-3). Additionally, Oct-2 regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. |
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Western blot - Oct2 antibody [EPR542]; Anti-Oct2 antibody [EPR542] at 1/1000 dilution + Daudi lysate at 10 µg.Secondary.HRP conjugated goat anti-rabbit antibody at 1/2000 dilution.Predicted band size : 51 kDa.Observed band size : 58 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Oct2 antibody [EPR542]; ab92458, at 1/250 staining Oct2 in Human tonsil paraffin embeeded section by immunohistochemistry.
Flow Cytometry-Anti-Oct2 antibody [EPR542](ab92458); Overlay histogram showing Raji cells stained with ab92458 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.