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Home Antibody All anti-POU2F2 antibodies

Anti-POU2F2 Antibody EPR542

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307420
  • Rabbit monoclonal antibody against 40088(clone EPR542 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419164) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
FC(1)
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Also for POU2F2 (NM_002698)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Oct.-2 was used as an immunogen.
Clone NameEPR542 IsotypeIgG
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsFC: 1:50, ICC: 1:250 - 500, IHC: 1:250 - 500, WB: 1:1,000 - 5,000,
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens POU class 2 homeobox 2 (POU2F2), transcript variant 2
Alternative NameOct-2; OCT2; OTF2
Database LinkNP_002689
FunctionOctamer transcription factor-2 (Oct-2) is a transcription factor that specifically binds to the octamer motif (5- ATTTCAT-3). Mostly expressed in B cells, Oct-2 posses a leucine zipper domain and belongs to the POU family of transcription factors (1-3). Additionally, Oct-2 regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression.
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Oct2 antibody [EPR542]; Anti-Oct2 antibody [EPR542] at 1/1000 dilution + Daudi lysate at 10 µg.Secondary.HRP conjugated goat anti-rabbit antibody at 1/2000 dilution.Predicted band size : 51 kDa.Observed band size : 58 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Oct2 antibody [EPR542]; TA307420, at 1/250 staining Oct2 in Human tonsil paraffin embeeded section by immunohistochemistry.
FC Image
Flow Cytometry-Anti-Oct2 antibody [EPR542](TA307420); Overlay histogram showing Raji cells stained with TA307420 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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