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Anti-PXN PHOSPHO Antibody EP41(2)Y
|A synthetic phospho-peptide corresponding to residues surrounding Tyr181 of human Paxillin was used as immunogen. The antibody only detects Paxillin phosphorylated on Tyrosine 181.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; FC: 1:10 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse
|Homo sapiens paxillin (PXN), transcript variant 4|
|Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4). |
EGFR1 Signaling Pathway
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Western blot - Paxillin (phospho Y181) antibody [EP41(2)Y]; All lanes : Anti-Paxillin (phospho Y181) antibody [EP41(2)Y] at 1/10000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HeLa cell lysate treated with pervanadate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 64 kDa.Observed band size : 68 kDa .