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Home Antibody All anti-SOX2 antibodies

Anti-SOX2 Antibody EPR3131

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307386
  • Rabbit monoclonal antibody against SOX2 (clone EPR3131 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401083) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(9)
ELISA(1)
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Also for SOX2 (NM_003106)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human SOX2 was used as an immunogen.
Clone NameEPR3131 IsotypeTissue culture supernatant
Species ReactivityMouse, Human, Leucoraja erinacea (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ELISA, IHC Suggested DilutionsWB: 1:1000 - 1:10000; ICC: 1:50 - 1:100; Sandwich ELISA: Use a concentration of 0.5 ug/ml; IHC-P: 1:100 - 1:250; IHC - Wholemount: Use at an assay dependent concentration; ICC/IF: Use at an assay dependent concentration
BufferDoes not react with Rat. Is unsuitable for IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens SRY (sex determining region Y)-box 2 (SOX2)
Alternative NameANOP3; MCOPS3
Database LinkNP_003097
Entrez Gene 6657 Human
Entrez Gene 20674 Mouse
FunctionSOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. It is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach (1). SOX2 is necessary for regulating multiple transcription factors that affect Oct3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct3/4 expression (2).
Related PathwayES Cell Differentiation/IPSAdult stem cellsCancer stem cellsEmbryonic stem cellsInduced pluripotent stem cellsTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - SOX2 antibody [EPR3131]; All lanes : Anti-SOX2 [EPR3131] antibody at 1/5000 dilution.Lane 1 : NCCIT cell lysate.Lane 2 : MCF-7 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 34 kDa.
ELISA Image
Sandwich ELISA - SOX2 antibody [EPR3131]; Standard Curve for SOX2 (Analyte: ; dilution range 1pg/ml to 1ug/ml using Capture Antibody at 0.2ug/ml and Detector Antibody at 0.5ug/ml.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody [EPR3131]; TA307386 at 1/100 dilution staining SOX2 in (1) glioma tissue, (2) Human adult brain tissue & (3) Human fetal brain tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SOX2 antibody [EPR3131](TA307386); TA307386 showing negative staining in Seminoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SOX2 antibody [EPR3131](TA307386); TA307386 showing positive staining in Breast carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131]; TA307386 showing negative staining in Normal lung tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SOX2 antibody [EPR3131](TA307386); TA307386 showing positive staining in Fetal stomach tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131]; TA307386 showing positive staining in Fetal lung tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131]; TA307386 showing positive staining in Embryonal carcinoma tissue.
IHC Image
IHC - Wholemount - Anti-SOX2 antibody [EPR3131]; IHC-Wholemount image of anti-Sox2 staining on a mouse blastocyst. This blastocyst was stained with anti-Sox2 antibody (pink) at a 1:200 dilution for 48 hours at 4oC. Nuclei stained with DAPI (grey).
IHC Image
IHC - Wholemount - Anti-SOX2 antibody [EPR3131]; TA307386 at 1/200 dilution staining SOX2 inIHC wholemount ofLeucoraja erinacea embryo.Sample was incubated with antibody for 48hours at 4°C in 10% fetal calf serum in PBT.Detection: DAB

 

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