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Anti-VIM Antibody EPR868(2)
|A synthetic peptide corresponding to residues in human Vimentin was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; ICC: 1:500 - 1:1000
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens vimentin (VIM)|
|Vimentin is the most common member of the intermediate filament (IF) family and one of the main components in the cytoskeleton structure. It is expressed during cell development and differentiation in a variety of mesenchymally and mesodermally derived cells (1). Vimentin is essential in cell integrity and cytoskeletal stability. The reorganization of vimentin, similar to all IF proteins, occurs during different stages of the cell cycle and cell signaling by a site-specific phosphorylation (serine and threonine residues) (2). Vimentin interacts with other proteins such as uridine phosphorylase and desmoplakin (3, 4). It is highly expressed in fibroblasts and many hormone-independent mammary carcinoma cell lines (5).|
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Western blot - Anti-Vimentin antibody [EPR868(2)]; All lanes : Anti-Vimentin antibody [EPR868(2)] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : A549 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution.Predicted band size : 53 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR868(2)]; Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Vimentin with ab133260 at 1/250 dilution.