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Home Antibody All anti-VIM antibodies

Anti-VIM Antibody EPR868(2)

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307359
  • Rabbit monoclonal antibody against Vimentin(clone EPR868(2))
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401165) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
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Also for VIM (NM_003380)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Vimentin was used as an immunogen.
Clone NameEPR868(2) IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; ICC: 1:500 - 1:1000
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.
Purification pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens vimentin (VIM)
Alternative NameCTRCT30; HEL113
Database LinkNP_003371
FunctionVimentin is the most common member of the intermediate filament (IF) family and one of the main components in the cytoskeleton structure. It is expressed during cell development and differentiation in a variety of mesenchymally and mesodermally derived cells (1). Vimentin is essential in cell integrity and cytoskeletal stability. The reorganization of vimentin, similar to all IF proteins, occurs during different stages of the cell cycle and cell signaling by a site-specific phosphorylation (serine and threonine residues) (2). Vimentin interacts with other proteins such as uridine phosphorylase and desmoplakin (3, 4). It is highly expressed in fibroblasts and many hormone-independent mammary carcinoma cell lines (5).
Related Pathway

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WB Image
Western blot - Anti-Vimentin antibody [EPR868(2)]; All lanes : Anti-Vimentin antibody [EPR868(2)] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : A549 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution.Predicted band size : 53 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR868(2)]; Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Vimentin with TA307359 at 1/250 dilution.

 

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