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Home Antibody All anti-SQSTM1 antibodies

Anti-SQSTM1 Antibody EPR4844

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Specifications Citations (1) Related Products Product Documents
SKU Description Amount Price Availability*  
TA307334
  • Rabbit monoclonal antibody against Sequestosome-1(clone EPR4844)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401285) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IF(1)
FC(1)
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Also for SQSTM1 (NM_003900)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Sequestosome-1 was used as an immunogen.
Clone NameEPR4844 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF, FC Suggested DilutionsWB: 1:10000 - 1:50000; ICC: 1:100 - 1:500; FC: 1:10 - 1:100
Predicted MW Explanation 62 kDa
BufferPreservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA

Reference Data

Target NameHomo sapiens sequestosome 1 (SQSTM1), transcript variant 1
Alternative NameA170; OSIL; p60; p62; p62B; PDB3; ZIP3
Database LinkNP_003891
Entrez Gene 8878 Human
Entrez Gene 18412 Mouse
Entrez Gene 113894 Rat
FunctionSequestosome-1 is a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. It functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Mutations in Sequestosome-1 result in sporadic and familial Paget disease of bone.
Related PathwayTranscription FactorsDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - SQSTM1 / p62 antibody [EPR4844]; All lanes : Anti-SQSTM1 / p62 antibody [EPR4844].Lane 1 : MCF-7.Lane 2 : HeLa.Lane 3 : SKBR-3.Lane 4 : 293T.Lysates/proteins at 10 ug per lane.Observed band size : 62 kDa .
IF Image
Immunocytochemistry/ Immunofluorescence - SQSTM1 / p62 antibody [EPR4844]; TA307334 staining of SQSTM1/p62 by immunofluorescence
FC Image
Flow Cytometry - Anti-SQSTM1 / p62 antibody [EPR4844]; Overlay histogram showing HeLa cells stained with TA307334 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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