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Anti-AP2M1 PHOSPHO Antibody EPR4700
Also for AP2M1 (NM_004068)
|A phospho specific peptide corresponding to residues surrounding Threonine 156 of human AP2 mu1 was used as an immunogen. This antibody only detects AP2 mu1 phosphorylated at Threonine 156.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; ICC: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,IHC-P or IP.
|Homo sapiens adaptor-related protein complex 2, mu 1 subunit (AP2M1), transcript variant 1|
|AP50; CLAPM1; mu2|
|Clathrin-coated vesicles, involved in endocytosis and Golgi processing, have a surface lattice containing clathrin triskelia and stoichiometric amounts of additional components termed "assembly proteins," or APs. The AP form at the plasma membrane, AP2, is composed of two large subunits of 100-115 kDa, denoted AP2 alpha and AP2 beta, a medium chain of 50 kDa, designated AP50, and a small chain (1). The medium chain AP2 mu1 interacts specifically with the tyrosine-based signals of several integral membrane proteins through the consensus sequence YXXPhi, where X can be any residue and Phi is a large hydrophobic residue (2). Phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P(2)] is an important factor for a variety of cellular functions ranging from cell signaling to actin cytoskeletal dynamics and endocytic membrane traffic. It has been shown that the clathrin adaptor complex AP2 acts as a regulator of phosphatidylinositol 4-phosphate 5-kinase (PIPK)-mediated PI(4,5)P(2) synthesis. AP2 directly interacts with the kinase core domain of type I PIPK isozymes via its mu2-subunit in vitro and in native protein extracts (3).|
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Western blot - AP2M1 (phospho T156) antibody [EPR4700]; All lanes : Anti-AP2M1 (phospho T156) antibody [EPR4700] at 1/1000 dilution.Lane 1 : 293T cell lysate .Lane 2 : 293T cell lysate treated with Calyculin A.Lysates/proteins at 10 µg per lane.Predicted band size : 50 kDa.