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Home Antibody All anti-AP2M1 antibodies

Anti-AP2M1 PHOSPHO Antibody EPR4700

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307326
  • Rabbit monoclonal antibody against AP2 mu1 Phospho (pT156)(clone EPR4700) (Phospho-Specific)
100ul 325 3-7 Days
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WB(1)

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding Threonine 156 of human AP2 mu1 was used as an immunogen. This antibody only detects AP2 mu1 phosphorylated at Threonine 156.
Clone NameEPR4700 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:1000 - 1:10000; ICC: 1:50 - 1:100
Predicted MW Explanation 50 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,IHC-P or IP.

Reference Data

Target NameHomo sapiens adaptor-related protein complex 2, mu 1 subunit (AP2M1), transcript variant 1
Alternative NameAP50; CLAPM1; mu2
Database LinkNP_004059
Entrez Gene 1173 Human
Entrez Gene 11773 Mouse
Entrez Gene 116563 Rat
FunctionClathrin-coated vesicles, involved in endocytosis and Golgi processing, have a surface lattice containing clathrin triskelia and stoichiometric amounts of additional components termed "assembly proteins," or APs. The AP form at the plasma membrane, AP2, is composed of two large subunits of 100-115 kDa, denoted AP2 alpha and AP2 beta, a medium chain of 50 kDa, designated AP50, and a small chain (1). The medium chain AP2 mu1 interacts specifically with the tyrosine-based signals of several integral membrane proteins through the consensus sequence YXXPhi, where X can be any residue and Phi is a large hydrophobic residue (2). Phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P(2)] is an important factor for a variety of cellular functions ranging from cell signaling to actin cytoskeletal dynamics and endocytic membrane traffic. It has been shown that the clathrin adaptor complex AP2 acts as a regulator of phosphatidylinositol 4-phosphate 5-kinase (PIPK)-mediated PI(4,5)P(2) synthesis. AP2 directly interacts with the kinase core domain of type I PIPK isozymes via its mu2-subunit in vitro and in native protein extracts (3).
Related PathwayDruggable Genome EndocytosisHuntington's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - AP2M1 (phospho T156) antibody [EPR4700]; All lanes : Anti-AP2M1 (phospho T156) antibody [EPR4700] at 1/1000 dilution.Lane 1 : 293T cell lysate .Lane 2 : 293T cell lysate treated with Calyculin A.Lysates/proteins at 10 ug per lane.Predicted band size : 50 kDa.

 

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