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Anti-UCHL1 Antibody EPR4117


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against PGP9.5(clone EPR4117)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401345) , 20ug Explanation
100ul $325 3-7 Days
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Also for UCHL1 (NM_004181)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human PGP9.5 was used as an immunogen.
Clone NameEPR4117 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:50 - 1:100; ICC: 1:50 - 1:100
Predicted MW Explanation 24 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) (UCHL1)
Alternative NameHEL-117; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; Uch-L1
Database LinkNP_004172
Entrez Gene 7345 Human
Entrez Gene 22223 Mouse
Entrez Gene 29545 Rat
FunctionPGP9.5 belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. It is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in PGP9.5 may be associated with Parkinson disease and Alzheimer's disease.
Related PathwayProteaseDruggable Genome Parkinson's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - PGP9.5 antibody [EPR4117]; All lanes : Anti-PGP9.5 antibody [EPR4117] at 1/1000 dilution.Lane 1 : Human fetal brain lysate.Lane 2 : Y79 lysate.Lane 3 : U-87 MG lysate.Lane 4 : SH SY5Y lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 24 kDa.
Western blot - Anti-PGP9.5 antibody [EPR4117]; All lanes : Anti-PGP9.5 antibody [EPR4117] at 1/1000 dilution.Lane 1 : Whole tissue lysate prepared from adult mouse brain homogenate (CD-1 strain).Lane 2 : Whole tissue lysate prepared from non transfected control cells (low endogenous expression of UCH-L1).Lane 3 : Whole tissue lysate prepared from cells transfected with UCH-L1-Flag cDNA (positive control) .Lysates/proteins at 100 ug per lane.Secondary.HRP conjugated goat anti-rabiit polyclonal at 1/10000 dilution.developed using the ECL technique.Predicted band size : 24 kDa.Exposure time : 2 minutes
Other-Anti-PGP9.5 antibody [EPR4117](TA307324); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PGP9.5 antibody [EPR4117]; TA307324 at 1/50 dilution staining PGP9.5 in Human brain by Immunohistochemistry, Paraffin-embedded tissue.


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