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Anti-UCHL1 Antibody EPR4118
Also for UCHL1 (NM_004181)
|A synthetic peptide corresponding to residues in human PGP9.5 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||ICC/IF: Use at an assay dependent concentration; WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; FC: 1:10 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC.
|Homo sapiens ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) (UCHL1)|
|NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; Uch-L1|
|PGP9.5 belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. It is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in PGP9.5 may be associated with Parkinson disease and Alzheimer’s disease.|
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Western blot - PGP9.5 antibody [EPR4118]; All lanes : Anti-PGP9.5 antibody [EPR4118] at 1/1000 dilution.Lane 1 : Fetal brain cell lysate.Lane 2 : Y79 cell lysate.Lane 3 : U87-MG cell lysate.Lane 4 : SH-SY5Y cell lysate.Lane 5 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit IgG at 1/2000 dilution.Predicted band size : 24 kDa.Observed band size : 25 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PGP9.5 antibody [EPR4118]; Immunohistochemical staining of PGP9.5 in paraffin embedded Human glioma tissue, using ab108986 at a 1/250 dilution.