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Home Antibody All anti-ARHGDIA antibodies

Anti-ARHGDIA Antibody EPR3772

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307307
  • Rabbit monoclonal antibody against Rho-GDI Alpha(clone EPR3772)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401371) , 20ug
100ul 325 3-7 Days
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WB(1)
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Also for ARHGDIA (NM_004309)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Rho-DGI alpha was used as an immunogen.
Clone NameEPR3772 IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:1000 - 1:5000; ICC: 1:50 - 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,IHC-P or IP.

Reference Data

Target NameHomo sapiens Rho GDP dissociation inhibitor (GDI) alpha (ARHGDIA), transcript variant 2
Alternative NameGDIA1; HEL-S-47e; NPHS8; RHOGDI; RHOGDI-1
Database LinkNP_004300
FunctionRho GDI constitutes a family with three mammalian members: RhoGDI alpha, the ubiquitously expressed archetypal member of the family; Ly/D4-GDI or RhoGDI beta, which has haematopoietic tissue-specific expression, particularly in B- and T-lymphocytes; and RhoGDI-3 or -gamma, which is membrane-anchored through an amphipathic helix and is preferentially expressed in brain, pancreas, lung, kidney and testis (1). RhoGDIs function by extracting Rho family GTPases including RhoA, Rac1, and Cdc42 from membranes and solubilizing them in the cytosol. Moreover, both in vitro and in vivo they interact only with prenylated Rho proteins. They also inhibit nucleotide exchange and GTP hydrolyzing activities on Rho proteins by interacting with their switch regions and probably restricting accessibility to GEFs and GAPs (2). The N-terminal domain of RhoGDI alpha binds to the switch region of the GTPases, affecting the GDP-GTP cycling, whereas the C-terminus accommodates the isoprenyl moiety of the GTPases in its hydrophobic pocket, regulating cytosol/membrane partitioning (3). RhoGDI alpha can be phosphorylated on two sites, Ser101 and Ser174, by PAK (P21-activated kinase) leading to selective activation of Rac1, but not RhoA or Cdc42, both in vitro and in vivo (4).
Related Pathway

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WB Image
Western blot - RhoGDI antibody [EPR3772]; All lanes : Anti-RhoGDI antibody [EPR3772] at 1/1000 dilution.Lane 1 : Jurkat cell lysates.Lane 2 : HeLa cell lysates.Lane 3 : HL-60 cell lysates.Lane 4 : NIH/3T3 cell lysates.Lysates/proteins at 10 µg per lane.Predicted band size : 23 kDa.Observed band size : 26 kDa .

 

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