OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-ERG antibodies

Anti-ERG Antibody EPR3864

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307295
  • Rabbit monoclonal antibody against ERG(clone EPR3864)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401413) , 20ug
100ul 379 In Stock
Add to Shopping Cart
WB(1)
IHC(8)
Assay(1)
spacer
Also for ERG (NM_004449)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C-terminus in human ERG was used as an immunogen. This antibody also detects Fli-1.
Clone NameEPR3864 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, ASSAY, IHC Suggested DilutionsICC: 1:100 - 250, IHC: 1:100 - 250, WB: 1:1,000 - 10,000,
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens v-ets avian erythroblastosis virus E26 oncogene homolog (ERG), transcript variant 2
Alternative Nameerg-3; p55
Database LinkNP_004440
FunctionTranscriptional Regulator ERG belongs to the ETS family that is involved in the regulation of a wide variety of cellular and developmental processes (1). This family of transcription factors contains approximately 30 members that share a highly conserved DNA-binding domain (ETS domain) (2, 3) and differs from each other in other domains (such as absence or presence of the Pointed/SAM domain) and are thus distinguished in sub-families (1). ERG belongs to the Erg/Fli-1 sub-family. It has been widely studied for its involvement in human cancers; TMPRSS2-ERG fusion, which occurs on account of translocations and interstitial deletions, is implicated in aggressive forms of prostate cancer (4, 5). ERG is linked to normal processes such as mesoderm formation and is found to form functional complexes with Jun/Fos, with the resulting ternary complexes regulating expression of genes such as metalloprotease-1 (MMP-1) and MMP-3 (1). It may also participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure (2).
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - ERG antibody [EPR3864]; Anti-ERG antibody [EPR3864] at 1/1000 dilution + Jurkat cell lysate at 10 µg.Secondary.HRP labelled Goat anti-Rabbit at 1/2000 dilution.Predicted band size : 55 kDa.
Assay Image
Other-Anti-ERG antibody [EPR3864](TA307295); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Frozen sections) - Anti-ERG antibody [EPR3864]; TA307295 stainingERG inHuman braintissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 3%serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in3% Horse serum) for 15 hours at 4°C. An undilutedHRP-conjugatedHorse anti-rabbit IgG polyclonal was used as the secondary antibody.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ERG antibody [EPR3864]; TA307295 at 1/100 dilution staining ERG in formalin-fixed, paraffin-embedded Human prostate adenocarcinoma tissue by Immunohistochemistry. Detection: DAB staining.
IHC Image
Immunohistochemistry (Frozen sections) - Anti-ERG antibody [EPR3864]; Immunohistochemical analysis of Fus A5 transgenic mouse prostate tissue, staining ERG with TA307295. Tissue sections were blocked in 20% goat serum, 2% BSA in PBS (GSB) for 40 min at room temperature, followed by an overnight incubation in primary antibody (1/25 in GSB) at 4°C. Following washes, the sections were incubated with a fluorescently tagged secondary antibody for 30 min at room temperature.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864]; Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using TA307295 showing -ve staining.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864]; Immunohistochemical analysis of paraffin embedded Human Hepatocellular carcinoma tissue using TA307295 showing -ve staining.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864]; Immunohistochemical analysis of paraffin embedded Human Prostatic adenocarcinoma stage 3 tissue using TA307295 showing +ve staining.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-ERG antibody [EPR3864](TA307295); Immunohistochemical analysis of paraffin embedded normal Human prostate tissue using TA307295 showing -ve staining.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-ERG antibody [EPR3864](TA307295); Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using TA307295 showing -ve staining.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title