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Home Antibody All anti-ERG antibodies

Anti-ERG Antibody EPR3864

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA307295
  • Rabbit monoclonal antibody against ERG(clone EPR3864)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401413) , 20ug Explanation
100ul $379 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(1)
IHC(8)
Assay(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C-terminus in human ERG was used as an immunogen. This antibody also detects Fli-1.
Clone NameEPR3864 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC Suggested DilutionsICC: 1:100 - 250, IHC: 1:100 - 250, WB: 1:1,000 - 10,000,
Predicted MW Explanation 55 kDa
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens v-ets avian erythroblastosis virus E26 oncogene homolog (ERG), transcript variant 2
Alternative Nameerg-3; p55
Database LinkNP_004440
Entrez Gene 2078 Human
Entrez Gene 13876 Mouse
Entrez Gene 170909 Rat
FunctionTranscriptional Regulator ERG belongs to the ETS family that is involved in the regulation of a wide variety of cellular and developmental processes (1). This family of transcription factors contains approximately 30 members that share a highly conserved DNA-binding domain (ETS domain) (2, 3) and differs from each other in other domains (such as absence or presence of the Pointed/SAM domain) and are thus distinguished in sub-families (1). ERG belongs to the Erg/Fli-1 sub-family. It has been widely studied for its involvement in human cancers; TMPRSS2-ERG fusion, which occurs on account of translocations and interstitial deletions, is implicated in aggressive forms of prostate cancer (4, 5). ERG is linked to normal processes such as mesoderm formation and is found to form functional complexes with Jun/Fos, with the resulting ternary complexes regulating expression of genes such as metalloprotease-1 (MMP-1) and MMP-3 (1). It may also participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure (2).
Related PathwayTranscription FactorsDruggable Genome

* Availability is in business days
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Western blot - ERG antibody [EPR3864]; Anti-ERG antibody [EPR3864] at 1/1000 dilution + Jurkat cell lysate at 10 ug.Secondary.HRP labelled Goat anti-Rabbit at 1/2000 dilution.Predicted band size : 55 kDa.
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Other-Anti-ERG antibody [EPR3864](TA307295); Equilibrium disassociation constant (KD)..
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Immunohistochemistry (Frozen sections) - Anti-ERG antibody [EPR3864]; TA307295 stainingERG inHuman braintissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 3%serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in3% Horse serum) for 15 hours at 4°C. An undilutedHRP-conjugatedHorse anti-rabbit IgG polyclonal was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ERG antibody [EPR3864]; TA307295 at 1/100 dilution staining ERG in formalin-fixed, paraffin-embedded Human prostate adenocarcinoma tissue by Immunohistochemistry. Detection: DAB staining.
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Immunohistochemistry (Frozen sections) - Anti-ERG antibody [EPR3864]; Immunohistochemical analysis of Fus A5 transgenic mouse prostate tissue, staining ERG with TA307295. Tissue sections were blocked in 20% goat serum, 2% BSA in PBS (GSB) for 40 min at room temperature, followed by an overnight incubation in primary antibody (1/25 in GSB) at 4°C. Following washes, the sections were incubated with a fluorescently tagged secondary antibody for 30 min at room temperature.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864]; Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using TA307295 showing -ve staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864]; Immunohistochemical analysis of paraffin embedded Human Hepatocellular carcinoma tissue using TA307295 showing -ve staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864]; Immunohistochemical analysis of paraffin embedded Human Prostatic adenocarcinoma stage 3 tissue using TA307295 showing +ve staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-ERG antibody [EPR3864](TA307295); Immunohistochemical analysis of paraffin embedded normal Human prostate tissue using TA307295 showing -ve staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-ERG antibody [EPR3864](TA307295); Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using TA307295 showing -ve staining.
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