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Home Antibody All anti-MCM2 antibodies

Anti-MCM2 PHOSPHO Antibody EP4120

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307290
  • Rabbit monoclonal antibody against MCM2 Phospho (pS53)(clone EP4120) (Phospho-Specific)
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding serine 53 of human MCM2 was used as an immunogen. This antibody only detects MCM2 phosphorylated at serine 53.
Clone NameEP4120 IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:10000 - 1:50000; IHC-P: 1:100 - 1:250; ICC/IF: 1:50 - 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens minichromosome maintenance complex component 2 (MCM2), transcript variant 1
Alternative NameBM28; CCNL1; cdc19; CDCL1; D3S3194; MITOTIN
Database LinkNP_004517
FunctionMinichromosome maintenance protein 2 (MCM2) is a member of the MCM family that regulates mammalian DNA replication (1). This family is composed of six related subunits, called the hexameric MCM2-7 complex, that are conserved in all eukaryotes. It functions as a replicative helicase, the molecular motor that both unwinds duplex DNA and powers fork progression during DNA replication (2). During cell division, MCM2 is required for the entry into S phase (3). It acts as a factor to license DNA for one and only one round of replication per cell cycle (3). MCM2 physically interacts with and is phosphorylated by Cdc7/Dbf4, thus regulating the initiation of DNA replication (4). Ser27, Ser41, and Ser139 are suggested to be Cdc7/Dbf4 phosphorylation sites in MCM2 (5).
Related Pathway
Cell cycle

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WB Image
Western blot - MCM2 (phospho S53) antibody [EP4120]; All lanes : Anti-MCM2 (phospho S53) antibody [EP4120] at 1/10000 dilution.Lane 1 : HeLa cell lysate untreated .Lane 2 : HeLa cell lysate treated with alkaline phosphatase (AP).Lysates/proteins at 10 µg per lane.Predicted band size : 102 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM2 (phospho S53) antibody [EP4120]; Immunohistochemical analysis of paraffin-embedded human tonsil tissue using TA307290 at a dilution of 1/100.
IF Image
Immunocytochemistry/ Immunofluorescence - MCM2 (phospho S53) antibody [EP4120]; Immunofluorescent staining of HeLa cells using TA307290 at a dilution of 1/50.

 

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