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Home Antibody All anti-MCM2 antibodies

Anti-MCM2 PHOSPHO Antibody EPR4119

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307284
  • Rabbit monoclonal antibody against MCM2 Phospho (pS27)(clone EPR4119) (Phospho-Specific)
100ul 325 3-7 Days
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WB(1)
IHC(2)
IF(1)

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding serine 27 of human MCM2 was used as an immunogen. This antibody only detects MCM2 phosphorylated at serine 27.
Clone NameEPR4119 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens minichromosome maintenance complex component 2 (MCM2), transcript variant 1
Alternative NameBM28; CCNL1; cdc19; CDCL1; D3S3194; MITOTIN
Database LinkNP_004517
FunctionMinichromosome maintenance protein 2 (MCM2) is a member of the MCM family that regulates mammalian DNA replication (1). This family is composed of six related subunits, called the hexameric MCM2-7 complex, that are conserved in all eukaryotes. It functions as a replicative helicase, the molecular motor that both unwinds duplex DNA and powers fork progression during DNA replication (2). During cell division, MCM2 is required for the entry into S phase (3). It acts as a factor to license DNA for one and only one round of replication per cell cycle (3). MCM2 physically interacts with and is phosphorylated by Cdc7/Dbf4, thus regulating the initiation of DNA replication (4). Ser27, Ser41, and Ser139 are suggested to be Cdc7/Dbf4 phosphorylation sites in MCM2 (5).
Related Pathway
Cell cycle

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - MCM2 (phospho S27) antibody [EPR4119]; All lanes : Anti-MCM2 (phospho S27) antibody [EPR4119] at 1/10000 dilution.Lane 1 : HeLa cell lysates untreated with alkaline phosphatase .Lane 2 : HeLa cell lysates treated with alkaline phosphatase .Lysates/proteins at 10 ug per lane.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 102 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM2 (phospho S27) antibody [EPR4119]; TA307284 at 1/100 dilution staining MCM2 (phospho S27) in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM2 (phospho S27) antibody [EPR4119]; TA307284 at 1/100 dilution staining MCM2 (phospho S27) in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
IF Image
Immunocytochemistry/ Immunofluorescence - MCM2 (phospho S27) antibody [EPR4119]; TA307284 at 1/100 dilution staining MCM2 (phospho S27) in HeLa cells by Immunofluorescence.

 

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