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Anti-LIMS1 Antibody EP1944


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against LIMS1(clone EP1944)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417616) , 20ug Explanation
100ul $325 3-7 Days
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human LIMS1 was used as an immunogen.
Clone NameEP1944 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250
Predicted MW Explanation 37 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens LIM and senescent cell antigen-like domains 1 (LIMS1), transcript variant 2
Alternative NamePINCH; PINCH-1; PINCH1
Database LinkNP_004978
Entrez Gene 3987 Human
Entrez Gene 110829 Mouse
Entrez Gene 499443 Rat
FunctionLIM and senescent cell antigen-like domains 1 (LIMS1, PINCH1) is an adaptor protein that contains five LIM domains. The LIM domain contains two zinc finger motifs that may direct protein-protein interactions (1, 2). LIMS1 is thought to be involved in integrin and growth factor signaling. During integrin signaling, LIMS1 interacts with ILK through its LIM domain. LIMS1 also acts as a bridge linking ILK to NCK2, assisting in growth factor signaling pathways. LIMS1 has been linked to coupling surface receptors to downstream signaling molecules involved in the regulation of cell differentiation, cell proliferation, and cell survival (3).
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - LIMS1 antibody [EP1944]; All lanes : Anti-LIMS1 antibody [EP1944] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : Jurkat cell lysate.Lane 4 : Human fetal kidney lysate.Lane 5 : Human spleen lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 37 kDa.Observed band size : 35 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LIMS1 antibody [EP1944]; Immunohistochemical staining of paraffin-embedded Human kidney tissue using TA307260 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - LIMS1 antibody [EP1944]; Immunofluorescent staining of HeLa cells using TA307260 at 1/100 dilution.


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