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Anti-XBP1 Antibody EPR4086
Also for XBP1 (NM_005080)
|A synthetic peptide corresponding to residues in human XBP1 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:500 - 1:1000; FC: 1:10 - 1:100; ICC/IF: 1:250 - 1:500
|Does not react with Mouse, Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens X-box binding protein 1 (XBP1), transcript variant 1|
|TREB5; XBP-1; XBP2|
|XBP1 is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. XBP1 may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of XBP1 is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5 (1).|
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Western blot - XBP1 antibody [EPR4086]; All lanes : Anti-XBP1 antibody [EPR4086] at 1/1000 dilution.Lane 1 : Fetal heart cell lysates .Lane 2 : Fetal kidney cell Fysates .Lane 3 : HT-29 cell lysates .Lane 4 : 293T cell lysates.Lane 5 : Jurkat cell lysates .Lysates/proteins at 10 µg per lane.Secondary.Standard HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 29 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XBP1 antibody [EPR4086]; Immunohistochemical analysis of paraffin-embedded Human colon tissue using ab109221 at 1/500.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XBP1 antibody [EPR4086]; Immunohistochemical analysis of paraffin-embedded Human urinary bladder transitional carcinoma tissue using ab109221 at 1/500.
Flow Cytometry - Anti-XBP1 antibody [EPR4086]; Overlay histogram showing HepG2 cells stained with ab109221 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.