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Anti-LMO2 Antibody EP3257
Also for LMO2 (NM_005574)
|A synthetic peptide corresponding to residues in human LMO2 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||FC: 1:100 - 500, IP: 1:100 - 500, WB: 1:1000 - 10,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Homo sapiens LIM domain only 2 (rhombotin-like 1) (LMO2), transcript variant 1|
|RBTN2; RBTNL1; RHOM2; TTG2|
|LMO2 (LIM-only protein 2, RBNT2), a member of the LIM-only class of transcriptional co-regulators that consist of two closely spaced cysteine-rich LIM domains has distinct functions in erythropoiesis and in T-cell leukemogenesis. These diverse functions of LMO2 are presumed to be accomplished through physical interaction with different with different protein partners that bind the LIM domains of LMO2 (1). It is also required for early stages of hematopoiesis, and the LMO2 master gene encodes a protein that has a central and crucial role in the hematopoietic development (2). LMO2 has been identified as a novel oncogene associated with carcinogenesis and prognosis in several malignant tumors (3)|
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Western blot - LMO2 antibody [EP3257]; All lanes : Anti-LMO2 antibody [EP3257] at 1/1000 dilution.Lane 1 : Raji cell lysate.Lane 2 : Fetal kidney lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 18 kDa.
Flow Cytometry - Anti-LMO2 antibody [EP3257]; Overlay histogram showing Ramos cells stained with ab91652 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.