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Anti-RBBP4 Antibody EPR3412
Also for RBBP4 (NM_005610)
|A synthetic peptide corresponding to residues in human RBBP4 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||FC: 1:50, ICC: 1:100 - 250, IHC: 1:250 - 500, IP: 1:50, WB: 1:10,000 - 20,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Rat
|Homo sapiens retinoblastoma binding protein 4 (RBBP4), transcript variant 1|
|RBBP4 is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. RBBP4 is a component of several complexes which regulate chromatin metabolism. These complexes include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair as well as the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression (1) The growth suppression function of the retinoblastoma protein (Rb) is thought to be mediated by Rb binding to cellular proteins. Rb interacts with multiple cellular proteins that mediate its cellular function. p48 is one of the major proteins that binds to a putative functional domain at the carboxy terminus of the Rb protein (2). Interaction with p48 may be one of the mechanisms for suppression of growth mediated by Rb. RbAp48 (RBBP4) and RbAp46 are quantitatively the major Rb-associated proteins. Results suggest that RBBP4 and RbAp46 may have shared as well as unique functions in the regulation of cell proliferation and differentiation (3). |
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Western blot - RbAp48 antibody [EPR3412]; All lanes : Anti-RbAp48 antibody [EPR3412] at 1/20000 dilution.Lane 1 : HeLa lysate.Lane 2 : Jurkat cell lysate.Lane 3 : Raji cell lysate.Lane 4 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 48 kDa.Observed band size : 48 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RbAp48 antibody [EPR3412]; ab92344 at 1/250 dilution staining RbAp48 in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.