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Anti-PDGFRA PHOSPHO Antibody EP2478
Also for PDGFRA (NM_006206)
|A phospho specific peptide corresponding to residues surrounding Tyrosine 720 of human PDGF Receptor alpha was used as an immunogen. This antibody only detects PDGF Receptor alpha phosphorylated at Tyrosine 720.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; FC: 1:500 - 1:1000
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC,IHC-P or IP.
|Homo sapiens platelet-derived growth factor receptor, alpha polypeptide (PDGFRA)|
|CD140A; PDGFR-2; PDGFR2; RHEPDGFRA|
|PDGF Receptor alpha is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development. Mice heterozygous for the receptor exhibit defective kidney phenotypes. Moreover, the SH2 domain of Shf can bind to PDGF Receptor alpha at tyrosine 720 (2).|
Cytokine-cytokine receptor interaction
MAPK signaling pathway
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Western blot - Anti-PDGF Receptor alpha (phospho Y720) antibody [EP2478]; All lanes : Anti-PDGF Receptor alpha (phospho Y720) antibody [EP2478] at 1/1000 dilution.Lane 1 : NIH 3T3 cell lysate, untreated.Lane 2 : NIH 3T3 cell lysate, treated with Pervanadate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 122 kDa.Observed band size : 190 kDa .
Flow Cytometry - Anti-PDGF Receptor alpha (phospho Y720) antibody [EP2478]; Flow Cytometry analysis of permeabilized NIH/3T3 cells labelling PDGF Receptor alpha (phospho Y720) with ab134068.Green - untreated, Red - pervanadate-treated, Blue - preincubated with phospho-PDGF Receptor alpha (phospho Y720) peptide, Orange - preincubated with non-phospho-PDGR Receptor alpha peptide.