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Anti-VCP Antibody EPR3307(2)
|A synthetic peptide corresponding to residues in human VCP was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; FC: 1:10 - 1:100; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens valosin containing protein (VCP)|
|ALS14; IBMPFD; IBMPFD1; p97; TERA|
|VCP (Valosin-containing protein) is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.|
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Western blot - VCP antibody [EPR3307(2)]; All lanes : Anti-VCP antibody [EPR3307(2)] at 1/10000 dilution.Lane 1 : MCF7 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : A549 cell lysate.Lane 4 : SH-SY5Y cell lysate.Lysates/proteins at 10 µg/ml per lane.Predicted band size : 89 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VCP antibody [EPR3307(2)]; Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab109240 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - VCP antibody [EPR3307(2)]; Immunofluorescent staining of HeLa cells using ab109240 at 1/100 dilution.
Flow Cytometry-Anti-VCP antibody [EPR3307(2)](ab109240); Overlay histogram showing HL60 cells stained with ab109240 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.