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Anti-GSTK1 Antibody EPR1939
Also for GSTK1 (NM_015917)
|A synthetic peptide corresponding to residues in human Gstk1was used as an immunogen.|
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||IHC-P: 1:250 - 1:500; WB: 1:1000 - 1:10000; IP: 1:30; FC: 1:60; ICC/IF: 1:250 - 1:500
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Does not react with Mouse, Rat
|Homo sapiens glutathione S-transferase kappa 1 (GSTK1), transcript variant 1|
|GST; GST 13-13; GST13; GST13-13; GSTK1-1; hGSTK1|
|Glutathione transferases (Gst) are a superfamily of enzymes that play a vital functional role in the cellular detoxification process. They catalyze the conjugation of the thiol group of glutathione to the electrophilic groups of a wide range of hydrophobic substrates, leading to an easier removal of the latter from the cells. The kappa class (Gstk) is the least studied one among various classes within the superfamily. The TRX-like domain of Gstk shares 19% sequence identity and structure similarity with the theta class Gst, suggesting that the kappa class is more closely related to the theta class enzyme within the Gst superfamily (1). Research has demonstrated that Gstk1 is expressed at relatively high levels in liver and stomach, and at moderate levels in kidney, heart, large intestine, testis, and lung. Sparse or essentially no Gstk1 protein was reported to be detected in small intestine, brain, spleen, and skeletal muscle (2). |
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Western blot - Anti-Glutathione S Transferase kappa 1 antibody [EPR1939]; All lanes : Anti-Glutathione S Transferase kappa 1 antibody [EPR1939] at 1/1000 dilution.Lane 1 : Human fetal liver lysate.Lane 2 : Human heart lysate.Lane 3 : 293T cell lysate.Lane 4 : HeLa cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 25 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione S Transferase kappa 1 antibody [EPR1939]; Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling Glutathione S Transferase kappa 1 with TA307095 at 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Glutathione S Transferase kappa 1 antibody [EPR1939]; Immunofluorescent analysis of HeLa cells labelling Glutathione S Transferase kappa 1 with TA307095 at 1/250 dilution.