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Home Antibody All anti-NDUFAF1 antibodies

Anti-NDUFAF1 Antibody EPR2796


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against NDUFAF1 (clone EPR2796 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC414253) , 20ug Explanation
100ul 325 In Stock
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Also for NDUFAF1 (NM_016013)
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues at the C terminus of human NDUFAF1 was used as an immunogen.
Clone NameEPR2796 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:10000 - 1:50000; IP: 1:40; ICC/IF: 1:100 - 1:250; IHC-P: 1:100 - 1:250
Predicted MW Explanation 38 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens NADH dehydrogenase (ubiquinone) complex I, assembly factor 1 (NDUFAF1), transcript variant 1
Alternative NameCGI-65; CGI65; CIA30
Database LinkNP_057097
Entrez Gene 51103 Human
Entrez Gene 69702 Mouse
Entrez Gene 296086 Rat
FunctionNDUFAF1 (CIA30) is a chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex 1) (1). Complex I is the largest multiprotein enzyme located in the inner mitochondrial membrane that catalyzes the transfer of electrons from NADH to coenzyme Q (CoQ). Little is known about the assembly process of the subunits into the mature complex, however, NDUFAF1 is one of two proteins found to be involved. NDUFAF1 expression is ubiquitous with a slightly higher expression in various heart tissues, kidney, lung and liver (2).
Related Pathway

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WB Image
Western blot - NDUFAF1 antibody [EPR2796]; All lanes : Anti-NDUFAF1 antibody [EPR2796] at 1/10000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : Human heart lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti Rabbit HRP-conjugated at 1/2000 dilution.Predicted band size : 38 kDa.Observed band size : 33 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NDUFAF1 antibody [EPR2796]; Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human liver tissue using 1/100 TA307092.
IF Image
Immunocytochemistry/ Immunofluorescence - NDUFAF1 antibody [EPR2796]; Immunofluorescence analysis of HeLa cells using 1/100 TA307092.


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