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Anti-NDUFAF1 Antibody EPR2796
Also for NDUFAF1 (NM_016013)
|A synthetic peptide corresponding to residues at the C terminus of human NDUFAF1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:10000 - 1:50000; IP: 1:40; ICC/IF: 1:100 - 1:250; IHC-P: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens NADH dehydrogenase (ubiquinone) complex I, assembly factor 1 (NDUFAF1), transcript variant 1|
|CGI-65; CGI65; CIA30|
|NDUFAF1 (CIA30) is a chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex 1) (1). Complex I is the largest multiprotein enzyme located in the inner mitochondrial membrane that catalyzes the transfer of electrons from NADH to coenzyme Q (CoQ). Little is known about the assembly process of the subunits into the mature complex, however, NDUFAF1 is one of two proteins found to be involved. NDUFAF1 expression is ubiquitous with a slightly higher expression in various heart tissues, kidney, lung and liver (2). |
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Western blot - NDUFAF1 antibody [EPR2796]; All lanes : Anti-NDUFAF1 antibody [EPR2796] at 1/10000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : Human heart lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti Rabbit HRP-conjugated at 1/2000 dilution.Predicted band size : 38 kDa.Observed band size : 33 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NDUFAF1 antibody [EPR2796]; Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human liver tissue using 1/100 ab79826.
Immunocytochemistry/ Immunofluorescence - NDUFAF1 antibody [EPR2796]; Immunofluorescence analysis of HeLa cells using 1/100 ab79826.