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Home Antibody All anti-LEF1 antibodies

Anti-LEF1 Antibody EP2030Y


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA307089 Rabbit monoclonal antibody against LEF-1/TCF-1 alpha (clone EP2030Y ) 100ul $325 In Stock
LC402529 LEF1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human LEF-1/TCF-1 alpha was used as an immunogen.
Clone NameEP2030Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsICC/IF: 1:100 - 1:250; WB: 1:25000 - 1:50000; FC: 1:20; IHC-P: Use at an assay dependent dilution
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens lymphoid enhancer binding factor 1 (LEF1), transcript variant 1
Alternative NameLEF-1; TCF10; TCF1ALPHA; TCF7L3
Database LinkNP_057353
Entrez Gene 51176 Human
FunctionThe human T cell-specific transcription factor TCF-1 alpha plays a key role in the tissue-specific activation of the T cell receptor (TCR) C alpha enhancer and binds to pyrimidine-rich elements (5'-PyCTTTG-3') present in a variety of other T cell-specific control regions. TCF-1 alpha is currently unique among the newly emerging family of DNA-binding regulatory proteins that share the HMG motif in that it is a highly tissue-specific RNA polymerase II transcription factor (1). TCF-1 alpha participates in the regulation of TCR alpha enhancer by facilitating the assembly of multiple proteins into a higher order nucleoprotein complex. The function of LEF-1 is dependent, in part, on the HMG domain that induces a sharp bend in the DNA helix, and on an activation domain that stimulates transcription only in a specific context of other enhancer-binding proteins (2). Constitutive activation of the Wnt signaling pathway is a root cause of many colon cancers. Activation of this pathway is caused by genetic mutations that stabilize the beta-catenin protein, allowing it to accumulate in the nucleus and form complexes with any member of the lymphoid enhancer factor and T-cell factor family of transcription factors (referred to collectively as LEF/TCFs) to activate transcription of target genes (3).
Related PathwayES Cell Differentiation/IPSAdult stem cellsTranscription FactorsDruggable Genome Wnt signaling pathwayAdherens junctionMelanogenesisPathways in cancerColorectal cancerEndometrial cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - LEF1 antibody [EP2030Y]; Anti-LEF1 antibody [EP2030Y] at 1/500000 dilution + Jurkat cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 44 kDa.Observed band size : 55 kDa .

IHC Image
Immunohistochemistry (Paraffin-embedded sections) - LEF1 antibody [EP2030Y]; Ab53293 (1:50) staining human LEF1 in human colon adenocarcinoma tissue by immunohistochemistry using paraffin embedded tissue.

FC Image
Flow Cytometry - LEF1 antibody [EP2030Y]; Flow cytometric analysis of permeabilized Jurkat cells using TA307089


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