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Anti-APBB1IP Antibody EP2806
Also for APBB1IP (NM_019043)
|A synthetic peptide corresponding to residues in human RIAM was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||ICC: 1:100 - 250, IHC: 1:100 - 250, IP: 1:50, WB: 1:2000 - 10,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Rat
|Homo sapiens amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein (APBB1IP)|
|INAG1; PREL1; RARP1; RIAM|
|RIAM (a Rap1-GTP-interacting adapter molecule) defines a family of adaptor molecules that contain a RA-like (Ras association) domain, a PH (pleckstrin homology) domain, and various proline-rich motifs (1). RIAM directly binds to Ena/VASP proteins, important modulators of cell migration that localize to focal adhesions and stress fibers, and co-localizes with them at focal adhesions in response to Ras activation. Moreover, RIAM directly binds to activated Ras in a phosphoinositide-dependent manner. RIAM is believed to be the first direct link between Ras signalling and cytoskeletal remodelling via Ena/VASP proteins during cell migration and spreading (2). |
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Western blot - RIAM antibody [EP2806]; All lanes : Anti-RIAM antibody [EP2806] at 1/2000 dilution.Lane 1 : Human Lymph node lysate.Lane 2 : Fetal brain lysate.Lane 3 : Human spleen lysate.Lane 4 : Jurkat cell lysate.Lane 5 : HL-60 + retinoic acid cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 73 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RIAM antibody [EP2806]; TA307075, at 1/100 dilution, staining RIAM in paraffin-embedded Human tonsil tissue by Immunohistochemistry.