OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-HLA-DRA antibodies

Anti-HLA-DRA Antibody EPR3692

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307072
  • Rabbit monoclonal antibody against HLA-Dra (clone EPR3692 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC412738) , 20ug
100ul 325 3-7 Days
Add to Shopping Cart
WB(1)
IHC(4)
FC(1)
Assay(1)
spacer
Also for HLA-DRA (NM_019111)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human HLA-Dra was used as an immunogen.
Clone NameEPR3692 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, FC Suggested DilutionsFC: 1:50; WB: 1:5000 - 1:20000; IP: 1:50; IHC-P: 1:100 - 1:250; ICC: 1:250 - 1:500
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens major histocompatibility complex, class II, DR alpha (HLA-DRA)
Alternative NameHLA-DRA1; MLRW
Database LinkNP_061984
FunctionHuman class II major histocompatibility complex (MHC) products are essential initiators of cellular immune responses. Their cell surface expression demonstrates aspects of tissue-restriction, inducibility, differentiation-dependence, and coordinate regulation indicative of complex molecular controls mediated primarily at the level of transcription (1). There are three major isotypes of human class II MHC molecules; HLA-DR, HLA-DP, and HLA-DQ, each of which consists of an alpha and beta chain (2). HLA-DR alpha is a polymorphic cell surface glycoprotein that is crucial for the cellular interaction in immune response. Class II molecules have limited tissue distribution and are predominantly expressed on B lymphocytes and macrophage and present peptides derived from extracellular proteins to T cells (3).
Related Pathway
Hematopoietic cell lineage

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - HLA-DRA antibody [EPR3692]; All lanes : Anti-HLA-DR antibody [EPR3692] at 1/5000 dilution.Lane 1 : Raji cell lysate.Lane 2 : Human spleen lysate.Lane 3 : Hu T-78 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 29 kDa.
Assay Image
Other-Anti-HLA-DR antibody [EPR3692](TA307072); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HLA-DRA antibody [EPR3692]; TA307072, at a 1/100 dilution, staining HLA-DRA in paraffin embedded Human tonsil (A) and Human kidney (B) tissue by Immunohistochemistry. Detection: by DAB staining
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-HLA-DR antibody [EPR3692](TA307072); TA307072 showing positive staining in Normal liver vessels tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-HLA-DR antibody [EPR3692](TA307072); TA307072 showing positive staining in Normal skin vessels tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-HLA-DR antibody [EPR3692](TA307072); TA307072 showing positive staining in Endometrial carcinoma vessels tissue.
FC Image
Flow Cytometry-Anti-HLA-DR antibody [EPR3692](TA307072); Overlay histogram showing Raji cells stained with TA307072 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title