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Anti-XRCC5 Antibody EPR3467
Also for XRCC5 (NM_021141)
|A synthetic peptide corresponding to residues in human Ku80 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||FC: 1:150, ICC: 1:100 - 250, IHC: 1:250 - 500, WB: 1:500 - 2000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) (XRCC5)|
|KARP-1; KARP1; KU80; Ku86; KUB2; NFIV|
|The Ku antigen is a DNA-associated nuclear protein originally recognized by the sera of patients with autoimmune diseases (1). It plays a key role in multiple nuclear processes, such as DNA repair, chromosome maintenance, transcription regulation, and V(D)J recombination (2). The Ku protein is composed of 70 and 82 kDa subunits (Ku70 and Ku80, respectively) and contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway (3). When bound to DNA, the Ku 70/80 heterodimer enhances the kinase activity of the catalytic subunit of the DNA-dependent protein kinase, DNA-PKcs (4). |
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Western blot - Ku80 antibody [EPR3467]; All lanes : Anti-Ku80 antibody [EPR3467] at 1/2000 dilution.Lane 1 : A549 cell lysate, treated with FBS .Lane 2 : HeLa cell lysate .Lane 3 : U937 cell lysate .Lane 4 : HepG2 cell lysate .Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 83 kDa.Observed band size : 83 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ku80 antibody [EPR3467]; ab79391 at 1/250 dilution staining Ku80 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - Anti-Ku80 antibody [EPR3467]; ab79391 (1/200) staining Ku80 in Hela cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
Flow Cytometry - Anti-Ku80 antibody [EPR3467]; Overlay histogram showing HeLa cells stained with ab79391 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.