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Anti-EGLN1 Antibody EPR3661
Also for EGLN1 (NM_022051)
|A synthetic peptide corresponding to residues on the C-terminus in human PHD2 was used as an immunogen.|
|Mouse, Human (Does not react with: Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||ICC/IF: 1:200; WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:50 - 1:100; ICC: 1:100 - 1:250; FC: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Rat
|Homo sapiens egl-9 family hypoxia-inducible factor 1 (EGLN1)|
|C1orf12; ECYT3; HALAH; HIF-PH2; HIFPH2; HPH-2; HPH2; PHD2; SM20; ZMYND6|
Entrez Gene 54583 Human
Entrez Gene 112405 Mouse
|PHD2 (Egl nine homolog 1) catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in PHD2 are associated with erythrocytosis familial type 3 (ECYT3).|
| Pathways in cancerRenal cell carcinoma|
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Western blot - PHD2 / prolyl hydroxylase antibody [EPR3661]; All lanes : Anti-PHD2 / prolyl hydroxylase antibody [EPR3661] at 1/1000 dilution.Lane 1 : SH-SY5Y cell lysate, treated with CoCl2.Lane 2 : SH-SY5Y cell lysate.Lane 3 : A549 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 46 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PHD2 / prolyl hydroxylase antibody [EPR3661]; TA307056 at 1/50 dilution staining PHD2 / prolyl hydroxylase in Human kidney by Immunohistochemistry, Paraffin-embedded tissue
ICC/IF image of TA307056 stained HepG2 cells. The cells were incubated with the antibody TA307056 at 1/200 dilution overnight at 4.. The secondary antibody (green) was DyLight? 488 goat anti- rabbit IgG (H+L) used at 1:250. for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Flow Cytometry - Anti-PHD2 / prolyl hydroxylase antibody; Overlay histogram showing SH-SY5Y cells stained with TA307056 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. This antibody gave a positive signal in SH-SY5Y cells under the same conditions.