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Anti-BRCC3 Antibody EPR4366
Also for BRCC3 (NM_024332)
|A synthetic peptide corresponding to residues in human BRCC36 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; FC: 1:100 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC or IHC-P.
|Homo sapiens BRCA1/BRCA2-containing complex, subunit 3 (BRCC3), transcript variant 1|
|BRCC36; C6.1A; CXorf53; RP11-143H17.2|
|BRCC36 is a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. It is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains, and does not have activity toward 'Lys-48'-linked polyubiquitin chains. In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs). This protein is also thought to be involved in the cellular response to ionizing radiation and progression through the G2/M checkpoint (1).|
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Western blot - BRCC36 antibody [EPR4366]; All lanes : Anti-BRCC36 antibody [EPR4366] at 1/10000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : MCF7 cell lysate.Lane 3 : SKBR-3 cell lysate.Lane 4 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 36 kDa.
Flow Cytometry - BRCC36 antibody [EPR4366]; Flow cytometric analysis of BRCC36 in permeabilized HeLa cells, using ab108411 at a 1/100 dilution (red) or a Rabbit IgG (negative) (green).