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Anti-BRCC3 Antibody EPR4365
Also for BRCC3 (NM_024332)
|A synthetic peptide corresponding to residues in human BRCC36 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; ICC: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IHC-P.
|Homo sapiens BRCA1/BRCA2-containing complex, subunit 3 (BRCC3), transcript variant 1|
|BRCC36; C6.1A; CXorf53; RP11-143H17.2|
|BRCC36 is a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. (1). It is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains, and does not have activity toward 'Lys-48'-linked polyubiquitin chains. In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs). BRCC36 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. It is aberrantly expressed in the vast majority of breast tumors (2). This protein is also thought to be involved in the cellular response to ionizing radiation and progression through the G2/M checkpoint (1).|
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Western blot - BRCC36 antibody [EPR4365]; All lanes : Anti-BRCC36 antibody [EPR4365] at 1/1000 dilution.Lane 1 : MCF7 cell lysate.Lane 2 : SKBR3 cell lysate.Lane 3 : 293T cell lysate.Lane 4 : Human fetal kidney lysate.Lane 5 : HeLa cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 36 kDa.