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Anti-KRAS Antibody EPR3255
Also for KRAS (NM_033360)
|A synthetic peptide corresponding to residues near the N-terminus of human K-Ras was used as an immunogen. This antibody also detects H-Ras and N-Ras.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; ICC: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or IHC-P.
|Homo sapiens Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant a|
|C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RASK2|
Entrez Gene 3845 Human
Entrez Gene 16653 Mouse
Entrez Gene 24525 Rat
|Ras, a signal transducer, was first characterized as the transforming genes of Harvey & Kristen sarcoma virus (1). The Ras family (H-Ras, N-Ras, and K-Ras) regulates cell growth, differentiation and apoptosis (2). Switching from an active or resting state, Ras can either bind GTP or GDP respectively. In the triphosphate conformation, Ras will interact with GTPase activating protein (GAP) to increase its activity (3). Mutations in any of the three isoforms can convert these proteins into active oncogenes. Additionally, Ras mutations are found in 30 % of all human cancer (4).|
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot - Ras antibody [EPR3255]; All lanes : Anti-Ras antibody [EPR3255] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : SH-SY5Y cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 22 kDa.