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Home Antibody All anti-EGLN2 antibodies

Anti-EGLN2 Antibody EPR2

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307021
  • Rabbit monoclonal antibody against PHD1(clone EPR2745)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC403281) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(2)
IF(1)
FC(1)
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Also for EGLN2 (NM_053046)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human PHD1 was used as an immunogen
Clone NameEPR2 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; FC: 1:10 - 1:100; ICC/IF: 1:100 - 1:250
Predicted MW Explanation 44 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens egl-9 family hypoxia-inducible factor 2 (EGLN2), transcript variant 1
Alternative NameEIT6; HIF-PH1; HIFPH1; HPH-1; HPH-3; PHD1
Database LinkNP_444274
Entrez Gene 112398 Human
Entrez Gene 112406 Mouse
Entrez Gene 308457 Rat
FunctionProlyl hydroxylase domain-containing protein 1 (PHD1, Hypoxia-inducible factor prolyl hydroxylase 1) is a 2-oxoglutarate and dioxygen-dependent enzyme that mediates the rapid destruction of hypoxia-inducible factor (HIF) alpha subunits by catalyzing their post-translational formations (1). PHD1 is in the Egln family of proline hydroxylases, functioning as a cellular oxygen sensor (2). Under normoxic conditions, it targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex (2). Loss of PHD1 has been shown to lower oxygen consumption in skeletal muscle by reprogramming glucose metabolism from oxidative to more anaerobic ATP production through the activation of a Pparalpha pathway (3).
Related PathwayDruggable Genome Pathways in cancerRenal cell carcinoma

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - PHD1/prolyl hydroxylase antibody [EPR2]; All lanes : Anti-PHD1/prolyl hydroxylase antibody [EPR2] at 1/1000 dilution.Lane 1 : A549 cell lysates .Lane 2 : HeLa cell lysates.Lane 3 : MCF-7 cell lysates .Lysates/proteins at 10 ug per lane.Secondary.Standard HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 44 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PHD1/prolyl hydroxylase antibody [EPR2]; Immunohistochemical analysis of paraffin-embedded Human ovarian adenocarcinoma tissue using TA307021
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PHD1/prolyl hydroxylase antibody [EPR2]; Immunohistochemical analysis of paraffin-embedded Human testis tissue using TA307021
IF Image
Immunocytochemistry/ Immunofluorescence - PHD1/prolyl hydroxylase antibody [EPR2]; Immunofluorescent staining of HeLa cells using TA307021
FC Image
Flow Cytometry-Anti-PHD1/prolyl hydroxylase antibody [EPR2](TA307021); Overlay histogram showing HeLa cells stained with TA307021 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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