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Anti-MSI2 Antibody EP1305Y
Also for MSI2 (NM_138962)
|A synthetic peptide corresponding to N-terminus of human Musashi2 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||ICC/IF: Use a concentration of 1 ug/ml; WB: 1:1000 - 1:2000; IHC-P: 1:100 - 1:250; FC: 1:30
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens musashi RNA-binding protein 2 (MSI2), transcript variant 1|
|Musahi2 (Msi2) is a member of Musashi family of evolutionarily conserved RNA-binding proteins. In mammals, Musahi1 and 2 shares similar primary structure, RNA binding specificity, and expression profile during CNS development. Highly co-expressed with Musahi1 in neural precursor cells, Musahi2 has been linked to proliferation and maintenance of stem cells in central nervous system. With two conserved tandem RNA recognition motifs, Musashi2 binds to target mRNAs and regulates RNA expression at translation level (1). Chromosomal aberrations involving Mushahi2 might play a role in disease progression for chronic myeloid leukemia (2). |
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Western blot - MSI2 antibody [EP1305Y]; All lanes : Anti-MSI2 antibody [EP1305Y] at 1/2000 dilution.Lane 1 : Rat brain cell lysates .Lane 2 : Human brain cell lysates .Lane 3 : SW480 cell lysates .Lane 4 : T47D cell lysates .Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 35 kDa.Observed band size : 35 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MSI2 antibody [EP1305Y]; Immunohistochemical analysis of paraffin-embedded human placenta with ab76148 at 1/100-1/250 dilution.
Immunocytochemistry/ Immunofluorescence-MSI2 antibody [EP1305Y](ab76148); ICC/IF image of ab76148 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Flow Cytometry-Anti-MSI2 antibody [EP1305Y](ab76148); Overlay histogram showing HeLa cells stained with ab76148 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.