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Anti-SKP1 Antibody EPR3303
Also for SKP1 (NM_170679)
|A synthetic peptide corresponding to residues in human SKP1 were used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||FC: 1:50, IHC: 1:100 - 250, WB: 1:1000 - 2000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens S-phase kinase-associated protein 1 (SKP1), transcript variant 2|
|EMC19; OCP-II; OCP2; p19A; SKP1A; TCEB1L|
Entrez Gene 6500 Human
Entrez Gene 287280 Rat
|SKP1 (S-phase kinase-associated protein 1) is a core component of the Skp1-Cullin-F-box (SCF) ubiquitin ligase protein complex. It mediates the ubiquitination of proteins involved in cell cycle progression, transcriptional regulation, and signal transduction. Among the components of the SCF complexes, SKP1 is an adaptor between one of the variable F-box proteins and Cullin (1). Independent of its function as a part of the SCF complex, SKP1 also functions in mitotic exit and cytokinesis. A possible contribution to the function of SKP1 in the mitotic exit is its physical interaction with Bfa1, a key negative regulator of the mitotic exit network (2). SKP1 also plays a crucial role in proper chromosomal segregation, which is required for the maintenance of euploidy and suppression of transformation (3). |
|Druggable Genome Cell cycleOocyte meiosisUbiquitin mediated proteolysisWnt signaling pathwayTGF-beta signaling pathway|
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Western blot - Skp1 antibody [EPR3303]; All lanes : Anti-Skp1 antibody [EPR3303] at 1/5000 dilution.Lane 1 : 293 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : PC12 cell lysate.Lane 4 : 3T3 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 19 kDa.Observed band size : 19 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Skp1 antibody [EPR3303]; Immunohistochemistry analysis of paraffin-embedded Human brain tissue with 1/100 TA307000.
Flow Cytometry - Skp1 antibody [EPR3303]; TA307000 at 1/50 dilution (red) staining Skp1 in permeabilized HeLa cells by Flow cytometry. Rabbit IgG (negative) is shown in green.