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Anti-CD8A Antibody EP1150
Also for CD8A (NM_171827)
|A synthetic peptide corresponding to residues near the C-terminus of human CD8 Alpha chain was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:5000; IP: 1:10; IHC-P: 1:250 - 1:500; ICC: 1:100 - 1:250; FC: 1:40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens CD8a molecule (CD8A), transcript variant 2|
|CD8; Leu2; MAL; p32|
|The T cell coreceptor CD8 is a cell-surface glycoprotein expressed either as a disulfide-linked homodimer of two CD8 alpha monomers, or a heterodimer of CD8 alpha and CD8 beta. These receptors interact with ligands, such as major histocompatibility complex (MHC) class I, on the outside of the cell, with proteins inside the cell, such as the tyrosine kinase p56lck, and possibly with proteins on the same cell-surface (1). CD8 plays an important role in T cell development in the thymus and T cell activation in the periphery (2).|
Hematopoietic cell lineage
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Western blot - CD8 alpha antibody [EP1150]; All lanes : Anti-CD8 alpha antibody [EP1150] at 1/1000 dilution.Lane 1 : MOLT-4 cell lysates .Lane 2 : Jurkat cell lysates .Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 26 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD8 alpha antibody [EP1150]; ab108343 at 1/250 dilution staining CD8 alpha in Human tonsil tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.