Anti-ITGA4 Antibody,

Also for ITGA4 (NM_000885)

OriGene Data

Immunogen	Integrin alpha 4 antibody was raised against a 15 amino acid peptide from near the center of human Integrin alpha 4.
Clone Name		Isotype	IgG
Species Reactivity	Human, Mouse, Rat	Concentration	1ug/ul
Guaranteed Application *	WB, IF	Suggested Dilutions	ELISA, WB: 1 ug/ml, IF
Buffer	PBS containing 0.02% sodium azide.
Purification	Affinity chromatography purified via peptide column (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) (ITGA4)
Alternative Name	CD49D; IA4
Database Link	NP_000876
Function	The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha 4 chain associates with either beta 1 or beta 7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha 4 beta 1 and alpha 4 beta 7 is located on the alpha 4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha 4 beta 7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha 4 beta 7, the gut mucosal homing receptor for peripheral T cells. Despite its predicted molecular weight, Integrin alpha 4 will often run at higher molecular weight in SDS-PAGE. At least two isoforms are known to exist. The immunogen to this antibody is within the Integrin alpha 4 recombinant protein fragment and thus will recognize it.
Related Pathway	Focal Adhesion Hematopoietic cell lineage Hemostasis

* Shipping is in business days

* OriGene provides validated application data and protocol, with money back guarantee.

WB Image

Western blot analysis of Integrin alpha 4 in rat spleen tissue lysate with Integrin alpha 4 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

IF Image

Immunofluorescence of Integrin alpha 4 in Jurkat cells with Integrin alpha 4 antibody at 2 ug/mL.

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-ITGA4 Antibody

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data