Home Antibody All anti-AES antibodies
|AES antibody was raised against a 16 amino acid peptide from near the amino-terminus of human AES.|
||ELISA, WB: 2 - 4 ug/ml, ICC: 10 ug/ml, IF: 20 ug/ml
|PBS containing 0.02% sodium azide.|
|Affinity chromatography purified via peptide column
|Homo sapiens amino-terminal enhancer of split (AES), transcript variant 2|
|AES-1; AES-2; ESP1; GRG; Grg-5; GRG5; TLE5|
Entrez Gene 166 Human
Entrez Gene 14797 Mouse
|Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (2,3). AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis (4). It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. (4). Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (4).|
|Transcription FactorsDruggable Genome |
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Western blot analysis of AES in 293 cell lysate with AES antibody at (A) 2 and (B) 4 ug/mL.
Immunocytochemistry of AES in 293 cells with AES antibody at 10 ug/mL.