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Home Antibody All anti-PTRH2 antibodies

Anti-PTRH2 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA306252
  • Rabbit Polyclonal Bit1 Antibody
  • FREE positive control: HEK293T cell transient overexpression lysate (LC414202) , 20ug
100ug 325 3-7 Days
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WB(1)
IHC(1)

OriGene Data

ImmunogenBit1 antibody was raised against a 16 amino acid peptide from near the center of human Bit1.
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse Concentration1ug/ul
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1 - 2 ug/ml
BufferPBS containing 0.02% sodium azide.
Purification Affinity chromatography purified via peptide column

Reference Data

Target NameHomo sapiens peptidyl-tRNA hydrolase 2 (PTRH2)
Alternative NameBIT1; CGI-147; PTH2
Database LinkNP_057161
FunctionAdhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process (2). Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (3). It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE (2). Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (2).
Related Pathway

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WB Image
Western blot analysis of Bit1 in Daudi cell lysate with Bit1 antibody at (A) 1, (B) 2, and (C) 4 ug/ml.
IHC Image
Immunohistochemistry of Bit1 in human small intestine tissue with Bit1 antibody at 10 ug/ml.

 

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