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Anti-CAMK2A Antibody EP1829Y
Also for CAMK2A (NM_015981)
|A synthetic peptide corresponding to residues on human CaMKII was used as an immunogen|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:20000; IP: 1:50; ICC: 1:250 - 1:500; FC: 1:80; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
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Western blot - CaMKII antibody [EP1829Y]; Anti-CaMKII antibody [EP1829Y] at 1/20000 dilution + Mouse brain lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 54 kDa.Observed band size : 45,70 kDa .
Immunohistochemistry (Paraffin-embedded sections) - CaMKII antibody [EP1829Y]; Immunohistochemical staining of paraffin-embedded human brain carcinoma using TA303876 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - CaMKII antibody [EP1829Y]; ICC/IF image of TA303876 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4Â°C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43?M.