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Anti-GABBR2 Antibody EP2411Y
Also for GABBR2 (NM_005458)
|A synthetic peptide corresponding to residues near the C-terminus of human GABA(B) R2 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:500; IHC: 1:100; ICC: 1:50; FC: 1:40
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens gamma-aminobutyric acid (GABA) B receptor, 2 (GABBR2)|
|GABABR2; GPR51; GPRC3B; HG20; HRIHFB2099|
|B-type receptors for the neurotransmitter GABA (gamma-aminobutyric acid) inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters and the activity of ion channels and adenylyl cyclase. See GABBR1 (MIM 603540) for additional background information on GABA-B receptors.[supplied by OMIM]. |
Signaling by GPCR
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Western blot - GABA B Receptor 2 antibody [EP2411Y]; Anti-GABA B Receptor 2 antibody [EP2411Y] at 1/500 dilution + human cerebellum lysate at 10 µg.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 106 kDa.Observed band size : 106 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GABA B Receptor 2 antibody [EP2411Y]; ab75838, at a 1/100 dilution, staining GABA B Receptor 2 in paraffin embedded human brain tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 2 antibody [EP2411Y]; Immunofluorescence analysis of GABA B Receptor 2 using ab75838 at 1/400 dilution. Left panel = normal rat DRG neurons. .Right panel = rat DRG neurons transfected with siRNAs to disrupt GABA B Receptor 2.
Flow Cytometry-Anti-GABA B Receptor 2 antibody [EP2411Y](ab75838); Overlay histogram showing SH-SY5Y cells stained with ab75838 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.