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Anti-MEF2A Antibody EP1706Y
Also for MEF2A (NM_005587)
|A synthetic peptide corresponding to residues near the N-terminal of human MEF2A protein was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:1000 - 1:10000; ICC: 1:100 - 1:250; FC: 1:20 - 1:50; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens myocyte enhancer factor 2A (MEF2A), transcript variant 1|
|ADCAD1; mef2; RSRFC4; RSRFC9|
|The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. The MEF2 genes are members of the MADS gene family (named for the yeast mating type-specific transcription factor MCM1, the plant homeotic genes 'agamous' and 'deficiens' and the human serum response factor SRF (MIM 600589)), a family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain.[supplied by OMIM]. |
TGF Beta Signaling Pathway
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Western blot - MEF2A antibody [EP1706Y]; All lanes : Anti-MEF2A antibody [EP1706Y] at 1/10000 dilution.Lane 1 : Fetal brain lysate.Lane 2 : Human heart lysate.Lane 3 : HeLa lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 55 kDa.Observed band size : 70 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MEF2A antibody [EP1706Y]; Immunohistochemical analysis of MEF2A in paraffin embedded human kidney tissue using ab76063 at a 1/50 dilution.
Flow Cytometry-MEF2A antibody [EP1706Y](ab76063); Overlay histogram showing MCF-7 cells stained with ab76063 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.